PCR Mutation Detection Protocols

Conformation Sensitive Capillary Electrophoresis (CSCE).- Conformation Sensitive Gel Electrophoresis.- Denaturing HPLC (dHPLC) for Mutation Screening.- In situ Detection of Human Papillomavirus DNA after PCR-Amplification.- LATE-PCR and Allied Technologies: Real-Time Detection Strategies for Rapid, Reliable Diagnosis from Single Cells.- Long PCR Amplification of Human Genomic DNA.- Human Papilloma Virus Strain Detection Utilizing Custom-Designed Oligonucleotide Microarrays.- Multiplex Ligation-Dependent Probe Amplification (MLPA®) for the Detection of Copy Number Variation in Genomic Sequences.- Screening for Genomic Rearrangements by Multiplex PCR/Liquid Chromatography (MP/LC).- Mutation Surveyor: Software for DNA Sequence Analysis.- Non-Invasive Prenatal Diagnosis (NIPD).- Automated DNA Sequencing.- Phylogenetic Microarrays for Cultivation-Independent Identification and Metabolic Characterization of Microorganisms in Complex Samples.- Prenatal Detection of Chromosome Aneuploidy by Quantitative-Fluorescence PCR.- Use of Robotics in High-Throughput DNA Sequencing.- Detection of Factor V Leiden and Prothrombin c.20210G>A Allele by Roche Diagnostics LightCycler®.- RT-PCR for the Detection of Translocations in Bone and Soft Tissue Tumors in Formalin-Fixed Paraffin-Embedded Tissues.- Detection of Minimal Residual Disease in Leukaemia by RT-PCR.- Mutation Detection by Southern Blotting.