Nuclear Mechanics and Genome Regulation

Dr. G. V. Shivashankar is currently the Deputy Director of Mechanobiology Institute, National University of Singapore. Shivashankar’s laboratory is focused on understanding the role of cell geometry on nuclear mechanics and genome regulation in living cells using a multi-disciplinary approach. He carried out his PhD research at the Rockefeller University (1994-1999) and Postdoctoral research at NEC Research Institute, Princeton, USA (1999-2000). He started his laboratory at the National Center for Biological Sciences, TIFR- Bangalore, India (2000-2009) before relocating to a tenured faculty position at the National University of Singapore in 2009. His scientific awards include the Birla Science Prize (2006) and the Swarnajayanthi Fellowship (2007), and he was elected to the Indian Academy of Sciences (2010). He also edited a Methods in Cell Biology volume on Nuclear Mechanics and Genome Regulation (2010), published by Elsevier. More recently, he headed the Joint Research Laboratory with the FIRC Institute of Molecular Oncology (IFOM), Milan, Italy and was appointed as an IFOM-NUS Chair Professor in 2014.

Section-A: Cell Nucleus: Organization and MechanoBiology



Fluorescence Fluctuation Microscopy to reveal 3D-Architecture and Function in the Cell Nucleus
Thorsten Lenser1,2, Klaus Weisshart2, Tobias Ulbricht3, Karolin Klement3, and Peter Hemmerich

Studying histone modifications and their genomic functions by employing chromatin immunoprecipitation and immunoblotting
Ranveer S. Jayani, Praveena L. Ramanujam and Sanjeev Galande

Dynamic organization of chromatin assembly & transcription factories in living cells
Bidisha Sinha, Dipanjan Bhattacharya, Deepak Kumar Sinha, Shefali Talwar,

Shovamayee Maharana, Soumya Gupta and G. V.Shivashankar

Manipulation & Isolation of Single Cells & Nuclei
Swee Jin Tan, Qingsen Li and Chwee Teck Lim

Beyond lamins: other structural components of the nucleoskeleton
Zhixia Zhong , Katherine L. Wilson and Kris Noel Dahl

Altered mechanical properties of the nucleus in disease
Maria Lucia Lombardi and Jan Lammerding

Theoretical concepts and models of cellular mechanosensing
Rumi De, Assaf Zemel and Samuel A. Safran

Section-B: Impact of Nuclear Mechanics on Function

Mechanical Induction of Gene Expression in Connective Tissue Cells
Mathew Chan, Boris Hinz, Christopher A. McCulloch

Physical plasticity of the Nucleus and its manipulation
Irena Ivanovska, Joe Swift, Takamasa Harada, J. David Pajerowski and Dennis E. Discher

Prestressed Nuclear Organization in Living Cells
Aprotim Mazumder, Roopa T. , Abhishek Kumar, K.Venkatesan Iyer,

Nisha M Ramdas and G. V.Shivashankar

Nanotopography/mechanical induction of stem-cell differentiation
Evelyn Yim

Mechanical Induction in Embryonic Development and Tumour Growth: Integrative Cues through Molecular to Multi-cellular Interplay, and Evolutionary Perspectives
Maria-Elena Fernandez-Sanchez, Fanny Serman, Padra Ahmadi and Emmanuel Farge

Informatics based analysis of mechanosignaling in the laminopathies
Frank P.L. Lai, Radfidah A. Mutalif, Siew Cheng Phua, and Colin L. Stewart

Autosomal Dominant Leukodystrophy caused by lamin B1 duplications: A clinical and molecular case study of altered nuclear function and disease

Quasar Saleem Padiath and Ying-Hui Fu