Stem Cell Labeling for Delivery and Tracking Using Noninvasive Imaging

Dara L. Kraitchman, VMD, PhD, FACC, is an associate professor in the Department of Radiology and Department of Molecular and Comparative Pathobiology at Johns Hopkins University. Her research interests include noninvasive imaging and minimally invasive treatment of cardiovascular disease. Joseph C. Wu, MD, PhD, is an associate professor in the Department of Medicine (Cardiology) and Department of Radiology at Stanford University School of Medicine. His research interests include stem cell biology, gene therapy, genomics, and molecular imaging.

Introduction: Stem Cell Types Overview and Rationale for Labeling for Imaging. Human Embryonic Stem Cells. Mesenchymal Stem Cells from Bone Marrow. Hematopoietic Stem Cells. Adipose-Derived Adult Stem Cells. Umbilical Cord Stem Cells. Induced Pluripotent Stem Cells. Radionuclide Approaches to Imaging Stem Cells and Their Biological Effects on the Myocardium. Fluorescence Imaging of Stem Cells In Vivo: Evolving Technologies and Applications. Ultrasound Imaging Basics. MRI Basics and Principles for Cellular Imaging. X-Ray Imaging Basics. Radionuclide Cell-Labeling Methods. Principles of Bioluminescence Imaging and Its Application In Vivo. Quantum Dot Labeling Methods. Magnetic Resonance Imaging Cell-Labeling Methods. Ultrasound Cell-Labeling Methods. X-Ray-Guided Delivery and Tracking of Cells. Multimodality/Fusion Imaging toward Imaging of Structure and Function. Other Non-Stem Cell Therapies for Cellular Tracking—Inflammatory Cell Tracking. Bioluminescence, MRI, and PET Imaging Modalities of Stem Cell-Based Therapy for Neurological Disorders. Clinical Application of Noninvasive Molecular Imaging in Cancer Cell Therapy: The First Reporter Gene-Based Imaging Clinical Trial. Clinical Cardiology Stem Cell Applications. Musculoskeletal Clinical Applications of Stem Cells. Regulatory Hurdles to Translation. Index.