DNA Topoisomerases

1. Introduction: Emerging Themes in DNA Topoisomerase Research.- 2. Analysis of DNA Topoisomers, Knots, and Catenanes by Agarose Gel Electrophoresis.- 3. Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers.- 4. Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II.- 5. Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA.- 6. Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy Transfer.- 7. Single-Molecule Magnetic Tweezers Studies of Type IB Topoisomerases.- 8. Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase III?.- 9. ChIP-on-Chip Analysis of DNA Topoisomerases.- 10. Analyzing Top2 Distribution on Yeast Chromosomes by Chromatin Immunoprecipitation.- 11. Binding of DNA Topoisomerases I and II to Replication Origins.- 12. Cytometric Assessment of DNA Damage Induced by DNA Topoisomerase Inhibitors.- 13. Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Human Cells.- 14. Assaying Topoisomerase II Checkpoints in Yeast.- 15. Cytological Analysis of Chromosome Structural Defects that Result from Topoisomerase II Dysfunction.- 16. Top2 SUMO Conjugation in Yeast Cell Lysates.- 17. Analysis of SUMOylation of Topoisomerase II? with Xenopus Egg Extracts.- 18. The Dynamics of DNA Topoisomerase II? in Living Cells.- 19. Depletion and Mutation of Topoisomerase II in Animal Cells.