Biochemical Principles and Techniques in Neuropharmacology

Buch | Softcover

298 Seiten

2012 | Softcover reprint of the original 1st ed. 1975
Springer-Verlag New York Inc.
978-1-4684-3167-4 (ISBN)

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It is difficult to imagine an era in which there were no selective drugs for treating anxiety, depression, schizophrenia, and other mental ailments. Yet in a remarkably short time these drugs have come to occupy a position of prominence in medical practice throughout the world, and they now account for a major portion of all prescriptions. Most psychotropic drugs were discovered with little premeditation on the part of the inves~ tigator. The drugs simply "worked," often with little rationale, and psychopharmacology has been for many years an empirical discipline in search of scientific underpinnings. In the past decade, a basic science of psychopharmacology has de veloped and grown rapidly. Though psychotropic drugs are exceedingly "young" as drugs in medical practice go, we probably know more about their various mechanisms of action than we know about most drugs in clinical use. Advances in understanding effects of psychopharmaceuticals on the brain have been so prodigious that a new research paradigm has evolved. Instead of being concerned solely with understanding how the drugs act, many researchers now employ psychotropic drugs as tools-often the most powerful ones-to elucidate brain function. Consequently, psychophar macology is central to neurobiology, which in turn has emerged as an important discipline, heir to the preeminent glamor of molecular biology.

1 Mass Fragmentography: A Tool for Studying Transmitter Function at Synaptic Level.- 1. Introduction.- 2. Technical Considerations.- 3. Biological Applications of Mass Fragmentography of Putative Neurotransmitters.- 4. Stable Isotopes and the Dynamic State of Neurotransmitters.- 5. Future Use of Mass Fragmentography.- 6. References.- 2 Notes on the Use of Gas-Liquid Chromatography Techniques for Determination of Psychotropic Drugs in Biological Samples.- 1. Introduction.- 2. Extraction of Drugs from Biological Samples.- 3. Derivatization.- 4. GC Conditions.- 5. Quantitative Analysis.- 6. Artifacts.- 7. Classification and List of Psychotropic Drugs Determined by Gas-Liquid Chromatography.- 8. References.- 3 Immunochemical Techniques in Neuropharmacology.- 4 A Practical Introduction to Radiometric Enzymatic Assays in Psychopharmacology.- 1. Introduction.- 2. Extraction of Enzyme from Nervous Tissue.- 3. Assay Conditions.- 4. Isolation of Radiolabeled Product.- 5. Sensitivity.- 6. Conclusion.- 7. References.- 5 Tissue Culture of the Nervous System: Applications in Neurochemistry and Psychopharmacology.- 1. Introduction.- 2. Tissue Culture: Some Definitions and Techniques.- 3. The Tissue Culture Laboratory.- 4. Media and Supplements.- 5. Organ Culture.- 6. Tissue Culture.- 7. Primary Cell Culture on Monolayer Surfaces.- 8. Primary Cell Culture as Aggregates.- 9. Cell Culture of Established Lines.- 10. Conclusions.- 11. Appendix: The Glass Shard Technique of Cloning.- 12. References.- 6 Chemical and Immunochemical Lesions by Specific Neurotoxic Substances and Antisera.- 1. Introduction.- 2. Peripheral Sympathectomy.- 3. Destruction of Central Catecholamine-Containing Fibers.- 4. Destruction of Serotonin-Containing Fibers in Brain.- 5. Conclusions.- 6. References.- 7 IsolatedNerve Terminals as an in Vitro Preparation for the Study of Dynamic Aspects of Transmitter Metabolism and Release.- 1. Introduction.- 2. Preparation of Synaptosomes.- 3. Metabolic Studies with Synaptosomes: Respiration and Energy Metabolism.- 4. Performance of Synaptosomes Under Depolarizing Influences.- 5. General Conclusions.- 6. References.- 8 Radiochemical Assay Procedures for Drugs and Transmitters.- 1. Introduction.- 2. Assays Based on the Formation of Labeled Derivatives by Enzymatic Procedures.- 3. Assays Based on the Formation of Labeled Derivatives by Isotopically Labeled Chemical Reagents.- 4. References.

Erscheint lt. Verlag	24.4.2012
Reihe/Serie	Handbook of Psychopharmacology ; 1
Reihe/Serie	Section I: Basic Neuropharmacology
Zusatzinfo	36 Illustrations, black and white; XIII, 298 p. 36 illus.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Geisteswissenschaften ► Psychologie
ISBN-10	1-4684-3167-6 / 1468431676
ISBN-13	978-1-4684-3167-4 / 9781468431674
Zustand	Neuware