Management of Chemical and Biological Samples for Screening Applications (eBook)

Mark Wigglesworth manages the UK Lead Optimization area of Sample Management Technologies for GlaxoSmith-Kline. Over the past 10 years Mark has managed large and small compound stores, assay ready plate production and compound set provision, as well as interacting with many areas of drug discovery. Additionally, he has led several drug discovery programs and overseen multiple screening platforms providing structure activity relationship data within GlaxoSmithKline?s screening organizations. Now a freelance business consultant, Terry Wood retired in 2011 as the manager of the Liquid Store Centre of Emphasis at Pfizer?s world-wide R&D department, based in Sandwich (UK). He has been involved in the high-throughput screening process for the whole of his 25 year career with Pfizer. This has given him a first hand and broad knowledge about the challenges and caveats of assay design, development and prosecution. His prime responsibility focused on the management of Pfizer?s extensive compound file, using a range of automated systems for sample preparation, storage and delivery.

PREFACE
INTRODUCTION TO SAMPLE MANAGEMENT

GENERATING A HIGH-QUALITY COMPOUND COLLECTION
Defining Current Screening Collections
Design Criteria for Enriching a Compound Collection with Drug-Like Compounds
Concluding Remarks

ASSESSING COMPOUND QUALITY
Introduction
Process Quality and Analytical Quality in Compound Management
Identity
Purity/Stability
Concentration/Solubility
Conclusions

DELIVERING AND MAINTAINING QUALITY WITHIN COMPOUND MANAGEMENT
Introduction
What is Quality from a Compound Management Perspective?
Storage and Delivery of Samples in Solution
Intercepting Low Purity
Storage and Delivery of Solids
Automation Quality Control and Reliability
High-Quality Data Management
Conclusion

OBTAINING AND MAINTAINING HIGH-QUALITY TISSUE SAMPLES: SCIENTIFIC AND TECHNICAL CONSIDERATIONS TO PROMOTE EVIDENCE-BASED BIOBANKING PRACTICE (EBBP)
Introduction
The Path toward Integration of Evidence-based Biobanking Practice
Integrating Evidence-based Biobanking Practice into Sample Protocols
Final Thoughts and Recommendations

THINKING LEAN IN COMPOUND MANAGEMENT LABORATORIES
The Emergence of 'Lean Thinking'
The Application of 'Lean Thinking'
Lean Thinking in Drug Discovery
A Lean Laboratory Toolbox
Streamlining Compound Processing - An Example
Summary


APPLICATION OF SUPPLY MANAGEMENT PRINCIPLES IN SAMPLE MANAGEMENT
Introduction
Common Pitfalls of Sample Management
Sample Management and Supply Chain Concepts
Implementing the Sample Management Strategy
Sample Management Organization
Sample Management Informatics
Avoid Monolithic Silos of Excellence
Position and Synchronize Inventory
Expand the Sample Management Boundary
Measuring and Assessing Effectiveness and Quality
Conclusions

SOLID SAMPLE WEIGHING AND DISTRIBUTION
The Practicalities and Technology of Weighing Solid Compounds
Logistical Challenges of Transportation of Small Molecules

MANAGING A GLOBAL BIOLOGICAL RESOURCE OF CELLS AND CELLULAR DERIVATIVES
Introduction
Diversity of Collections
Sourcing and Acquisition
Authentication and Characterization
Cryopreservation, Storage, and Production
Data Management
Quality and Standards
Order Fulfillment and Distribution
Offsite Biorepository Management
Regulatory and Legal Compliance
Ownership and Intellectual Property Management
Collaborations
Conclusion

DEVELOPMENT OF AUTOMATION IN SAMPLE MANAGEMENT
Introduction
Historical Background
Automation of Sample Management Today
System Building Blocks
Storage Systems
Liquid Handler
Accessories
Plate Handling, Integration
Case Study: Evolution of a Compound Management Group
Results

APPLICATIONS OF ACOUSTIC TECHNOLOGY
Introduction
Compound-Handling Challenges in Drug Discovery
Acoustic Drop Ejection - Performance, Quality Assurance, and Platform Validation
Acoustic-Assisted Compound Solubilization and Mixing
Acoustic Applications in Drug Discovery
Emerging Applications

ENHANCING BIOREPOSITORY SAMPLE INTEGRITY WITH AUTOMATED STORAGE AND RETRIEVAL
The Emerging Growth of Biobanking
Automated Storage and Retrieval in a Biorepository
Configuration of an Automated Biorepository
Conclusions

INFORMATION TECHNOLOGY SYSTEMS FOR SAMPLE MANAGEMENT
Sample Registration
Intellectual Property and Laboratory Notebooks
Some Observations on Information Technology
Biological Data Management

KEY FEATURES OF A COMPOUND MANAGEMENT SYSTEM
Why Do We Need Compound Management Information Technology Systems?
Compound Management Software
Benefits of Commercially Available Compound Management Systems

WHAT DOES AN HTS FILE OF THE FUTURE LOOK LIKE?
Introduction
History of Compounds Collection for HTS
Impact of High-Throughput Chemistry on Corporate Files
Chemical Library Management
The Concept of Drug-Likeness and the Lipinski Rules
Quality versus Quantity
The Emergence of the Subsets: Fragment, G-Protein-CoupledReceptor (GPCR), Ion Channel, Kinase, Protein-Protein Interaction, Chemogenomics, Library Of Pharmacological