Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis (eBook)

Acknowledgments 6
Contents 8
Introduction 10
1 Chorioallantoic Membrane Vasculature 12
1.1 Chorioallantoic Membrane and Its Embryological Origin 12
1.2 Morphology of Chorioallantoic Membrane Blood Vessels 14
1.3 Morphology of Chorioallantoic Membrane Lymphatic Vessels 21
1.4 A Single Blood Sinus or a Capillary Plexus Beneath the Chorionic Epithelium? 22
1.5 A New Model of Choriollantoic Membrane Vascular Growth, the Intussusceptive Mode 23
1.6 Chorioallantoic Membrane Vascular Growth 24
2 Chorioallantoic Membrane in the Study of Angiogenesis, Antiangiogenesis, and the Vascularization of Grafted Tissues 27
2.1 Use of Chorioallantoic Membrane in the Study of Angiogenic Molecules 27
2.2 Role of FGF-2 in Chorioallantoic Membrane Vascularization 33
2.3 Use of Chorioallantoic Membrane in the Study of Antiangiogenic Molecules 39
2.4 Use of Chorioallantoic Membrane in the Study of Vascularization of Grafted Tissues 49
3 Chorioallantoic Membrane in the Study of Tumor Angiogenesis 51
3.1 Use of Chorioallantoic Membrane in the Study of Tumor Angiogenesis 51
3.2 Angiogenesis and Antiangiogenesis in Multiple Myeloma 55
3.2.1 Biological and Clinical Studies 55
3.2.2 Use of the Chorioallantoic Membrane 56
3.3 Angiogenesis and Antiangiogenesis in Human Neuroblastoma 59
3.3.1 Biological and Clinical Studies 59
3.3.2 Use of Chorioallantoic Membrane 62
4 Chorioallantoic Membrane in the Study of Tumor Metastasis 68
4.1 Use of Chorioallantoic Membrane in the Study of Tumor Metastasis 68
4.2 Spontaneous Metastasis Models 69
4.3 Experimental Metastasis Studies 70
5 Other Applications of Chorioallantoic Membrane 73
5.1 In Ovo and Ex Ovo Methods 73
5.2 Use of Chorioallantoic Membrane in the Study of Tumor Lymphangiogenesis 74
5.3 Use of Chorioallantoic Membrane in the Study of Angiogenesis Associated with Wound Healing and Inflammation 74
5.4 Use of Chorioallantoic Membrane in the Study of Angiogenic Properties of Biomaterials and of the Evaluation of Drug Delivery Systems 76
6 Different Morphological Techniques and Methods of Quantifying the Angiogenic Response Used in the Study of Vascularization in the Chorioallantoic Membrane 78
6.1 Different Morphological Techniques That Can be Used to Study Vascularization of the CAM and the Genes Involved 78
6.2 Methods of Quantifying the Angiogenic Response 79
7 Advantages and Limitations of Chorioallantoic Membrane in Comparison with Other Classical In Vivo Angiogenesis Assays 82
7.1 Advantages and Limitations of Chorioallantoic Membrane Assay 82
7.2 Other Classical In Vivo Assays in Comparison (Advantages, Disadvantages, and Limitations) with Chorioallantoic Membrane 83
7.2.1 The Corneal Micropocket Assay 84
7.2.2 The Sponge/Matrix Implant 86
7.2.3 The Disk Angiogenesis System (DAS) 87
7.2.4 The Matrigel Plug 87
7.2.5 The Dorsal Air Sac Model 88
7.2.6 The Chamber Assays 89
7.2.7 The Zebrafish 89
7.2.8 The Tumor Models 91
Concluding Remarks 93
References 95