Fundamental Laboratory Approaches for Biochemistry and Biotechnology

Alexander J. Ninfa is Associate Professor in the Department of Biological Chemistry at the University of Michigan and is internationally recognized for his work on the biochemical mechanisms of signal transduction and transcriptional regulation in bacteria. David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.

Preface. Chapter 1: Basic Practices and Techniques in the Biochemistry Laboratory. Chapter 2: Spectrophotometry. Chapter 3: Quantification of Protein Concentration. Chapter 4: Chromatography. Chapter 5: Gel Electrophoresis of Proteins. Chapter 6: Overview of Protein Purification. Chapter 7: Isolation and Characterization of the Enzyme Alkaline Phosphatase From Escherichia Coli. Chapter 8: Enzyme Kinetics. Chapter 9: Enzymatic Methods of Analysis. Chapter 10: Ligand Binding. Chapter 11: Recombinant DNA Techniques. Chapter 12: Polymerase Chain Reaction (PCR) Technology. Chapter 13: Using the Personal Computer and the Internet for Biochemical Research. Appendix 1: Safety Checklist. Appendix 2: An Example of an MSDS. Index.