Yeast Metabolic Engineering

lt;p>Preface...
Table of Contents...
Contributing Authors...

1. An Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae
Verena Siewers

2. High Efficiency Plasmid DNA Transformation in Yeast
O'Taveon R. Fitzgerald, Nestor D. Rodriguez, and L. Kevin Lewis

3. CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae
Tadas Jakociunas, Michael K. Jensen, and Jay D. Keasling

4. Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening
Christos Skrekas, Raphael Ferreira, and Florian David

5. Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology
Kouichi Kuroda and Mitsuyoshi Ueda

6. Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris
Claudia Rinnofner, Michael Felber, and Harald Pichler

7. Competent Cell Preparation and Transformation of Pichia pastoris
Joan Lin-Cereghino, Christopher A. Naranjo, and Geoff P. Lin-Cereghino

8. CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome
Jasmin Elgin Fischer and Anton Glieder

9. Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris
Simon Kobalter, Astrid Radkohl, Helmut Schwab, Anita Emmerstorfer-Augustin, and Harald Pichler

10. Engineering of Promoters for Gene Expression in Pichia pastoris
Thomas Vogl

11. Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory
Paola Branduardi, Liliane Barroso, Laura Dato, Edward J. Louis, and Danilo Porro

12. Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica
Macarena Larroude, Jean-Marc Nicaud, and Tristan Rossignol

13. A Universal Gene Expression System for Novel Yeast Species
Dominik Majzita, Anssi Rantasalo, and Marja Ilmén

14. Dynamic Feeding of Pichia pastoris
Alexander Pekarsky and Oliver Spadiut

15. Automated Evolutionary Engineering of Yeasts
Erik de Hulster, Christiaan Mooiman, Rowin Timmermans, and Robert Mans

16. Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN
Francisco Zorrilla and Eduard J. Kerkhoven

Subject Index List...