Single Molecule Analysis

Introduction to Optical Tweezers: Background, System Designs, and Applications.- Quantifying ATP-Independent Nucleosome Chaperone Activity with Single Molecule Methods.- Protein Tethering for Single-Molecule Force Spectroscopy.- Insect Cell-Based Expression of Cytoskeletal Motor Proteins for Single-Molecule Studies.- Probing Mitotic Chromosome Mechanics Using Optical Tweezers.- A Brief Introduction to Single-Molecule Fluorescence Methods.- Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans.- Lattice Light-Sheet Motor-PAINT: A Method to Map the Orientations of Microtubules in Complex Three-Dimensional Arrays.- Fluorescence Microscopy of Nanochannel-Confined DNA.- Single-Molecule FRET X.- Single-Molecule Fluorescence Imaging of DNA Replication Stalling at Sites of Nucleoprotein Complexes.- Measuring Transcription Dynamics of Individual Genes Inside Living Cells.- Single-Molecule FRET Resolved Protein Dynamics from Plasmid to Data in Six Steps.- Atomic Force Microscopy: An Introduction.- Atomic Force Microscopy of Viruses: Stability, Disassembly, and Genome Release.- Unfolding and Refolding Proteins using Single-Molecule AFM.- Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy.- An Introduction to Magnetic Tweezers.- Surface Functionalization, Nucleic Acid Tether Characterization, and Force Calibration for a Magnetic Tweezers Assay.- Correlated Single-Molecule Magnetic Tweezers and Fluorescence Measurements of DNA-Enzyme Interactions.- Detecting DNA Loops Using Tethered Particle Motion.- Single-Cell Measurements using Acoustic Force Spectroscopy (AFS).- DNA Origami-Based Single-Molecule Force Spectroscopy and Applications.