Riboregulator Design and Analysis

RNP-Based Control Systems for Genetic Circuits in Synthetic Biology Beyond CRISPR.- Computational Design of RNA Toehold-Mediated Translation Activators.- Design of RNA-Based Translational Repressors.- Design of Ribocomputing Devices for Complex Cellular Logic.- Computational Design of Small Transcription Activating RNAs (STARs).- Design and Assembly of Multi-Level Transcriptional and Translational Regulators for Stringent Control of Gene Expression.- Model-Based Design of Synthetic Antisense RNA for Predictable Gene Repression.- Design of a Toolbox of RNA Thermometers.- Development of Synthetic Riboswitches to Guide the Evolution of Metabolite Production in Microorganisms.- Efficient Method to Identify Synthetic Riboswitches Using RNA-Based Capture-SELEX Combined with In Vivo Screening.- RNA Design Principles for Riboswitches that Regulate RNase P-Mediated tRNA Processing.- Design, Characterization, and Application of Targeted Gene Activation in Bacteria Using a Modular CRISPRa System.- Reprogramming TracrRNAs for In Vitro RNA Detection and In Vivo Transcriptional Recording.- Harnessing CRISPR-Cas9 for Epigenetic Engineering.- RNA Structure Prediction, Analysis, and Design: An Introduction to Web-Based Tools.- Single-Molecule FRET Studies of RNA Structural Rearrangements and RNA-RNA Interactions.- Cotranscriptional RNA Chemical Probing.