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Understanding Light Microscopy

J Sanderson (Autor)

Software / Digital Media

848 Seiten

2019
John Wiley & Sons Inc (Hersteller)
978-1-118-69673-6 (ISBN)

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This textbook introduces readers to the modern light microscope. It can either be used alone or in conjunction with a practical course. The book begins with an account of how the light microscope works, and how to set up the instrument for optimum performance. The nature of light, and its influence on the function of the microscope, as well as practical advice on selecting the most suitable components, is explained to the reader. An account of the different types of currently-available light microscopy modes follows, together with a guide, borne out by the authors practical experience, on how to choose between transmitted or reflected light microscopes; wide-field, confocal, deconvolution or multi-photon microscopes. A description of specimen preparation is given, as well as advice upon cameras, image acquisition and post-processing of data.
* Gives a didactic introduction to the light microscope * Explanation complemented with simple experiments where appropriate * Simple explanation (from teaching the subject) enables a self-taught student to start from scratch to set up and acquire high quality images * Leads the student on, should they wish, to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility * Well-illustrated in colour, the illustrations are selected (from long teaching experience) to support text * Workable practical protocols * Includes a chapter summary to introduce learning objectives Part of the Royal Microscopical Society co-publication book program

JEREMY SANDERSON is a Bio-Imaging specialist at the Mammalian Genetics Unit, MRC Harwell Institute, in the UK. He is Chartered Scientist and a Fellow of the Royal Microscopical Society and has served both on the Executive Council and the LM Section Committee.

About the Author

Acknowledgements

Glossary and definitions

About the Companion Website

Notes

Introduction

Chapter 1 Our eyes and the microscope

Chapter 2 Light

Chapter 3 Basic microscope optics

Chapter 4 Microscope design

Chapter 5 Ergonomics

Chapter 6 Optical aberrations of the microscope

Chapter 7 The microscope objective

Chapter 8 Condensers & eyepieces

Chapter 9 Illumination in the microscope

Chapter 10 Diffraction and image formation in microscopy

Chapter 11 Contrast generation & enhancement

Chapter 12 Reflected-light microscopy

Chapter 13 Polarised-light microscopy: part 1 - theory

Chapter 14 Polarised-light microscopy: part 2 - applied

Chapter 15 Fluorescence microscopy

Chapter 16 Fluorophores & fluorescent proteins

Chapter 17 Optical sectioning & confocal microscopy

Chapter 18 Operating the confocal microscope

Chapter 19 Light-sheet microscopy

Chapter 20 Bleed-through & Spectral unmixing

Chapter 21 Deconvolution

Chapter 22 Multi-photon microscopy

Chapter 23 Total Internal Reflection Fluorescence microscopy

Chapter 24 FRAP and FRET

Chapter 25 Colocalisation

Chapter 26 Super-resolution fluorescence microscopy

Chapter 27 - Choosing a microscope platform and Core Imaging Facilities

Chapter 28 Biological Specimen Preparation

Chapter 29 Materials Specimen Preparation

Chapter 30 Recording the image: part 1 - theory

Chapter 31 Recording the image: part 2 - applied

Appendices

1. Buying and tendering for a light microscope

2. Trouble-shooting poor image quality

3. The Michel-Levy Interference chart

4. Cleaning and Maintenance

5. Microscope Manufacturer contacts

6. Brief historical notes

7. Timeline

Index

Verlagsort	New York
Sprache	englisch
Maße	150 x 250 mm
Gewicht	666 g
Themenwelt	Naturwissenschaften ► Biologie
	Naturwissenschaften ► Chemie
	Technik ► Maschinenbau
ISBN-10	1-118-69673-5 / 1118696735
ISBN-13	978-1-118-69673-6 / 9781118696736
Zustand	Neuware