Ultra-High Performance Liquid Chromatography and Its Applications (eBook)

Explores both the benefits and limitations of new UHPLC
technology

High performance liquid chromatography (HPLC) has been widely
used in analytical chemistry and biochemistry to separate,
identify, and quantify compounds for decades. The science of liquid
chromatography, however, was revolutionized a few years ago with
the advent of ultra-high performance liquid chromatography (UHPLC),
which made it possible for researchers to analyze sample compounds
with greater speed, resolution, and sensitivity.

Ultra-High Performance Liquid Chromatography and Its
Applications enables readers to maximize the performance of
UHPLC as well as develop UHPLC methods tailored to their particular
research needs. Readers familiar with HPLC methods will learn how
to transfer these methods to a UHPLC platform and vice versa. In
addition, the book explores a variety of UHPLC applications
designed to support research in such fields as pharmaceuticals,
food safety, clinical medicine, and environmental science.

The book begins with discussions of UHPLC method development and
method transfer between HPLC and UHPLC platforms. It then examines
practical aspects of UHPLC. Next, the book covers:

* Coupling UHPLC with mass spectrometry

* Potential of shell particles in fast liquid chromatography

* Determination of abused drugs in human biological matrices

* Analyses of isoflavones and flavonoids

* Therapeutic protein characterization

* Analysis of illicit drugs

The final chapter of the book explores the use of UHPLC in drug
metabolism and pharmacokinetics studies for traditional Chinese
medicine.

With its frank discussions of UHPLC's benefits and limitations,
Ultra-High Performance Liquid Chromatography and Its
Applications equips analytical scientists with the skills and
knowledge needed to take full advantage of this new separation
technology.