Engineering Complex Phenotypes in Industrial Strains

RANJAN PATNAIK, PhD, is a chemical engineer by profession with 17 years of practical experience in the field of industrial biotechnology that includes small biotech start-ups and large enterprises. His research interests are in the field of metabolic engineering, fermentation optimization, process integration and R&D technology management. He currently heads the biofuels research group at DuPont Knowledge Center (DKC), Hyderabad, India. Prior to his current role, he had a variety of responsibilities within the biochemical sciences & engineering division of CR&D, E. I. du Pont de Nemours and Company, Wilmington, DE, USA.

Foreword John Pierce PREFACE Ranjan Patnaik CHAPTER 1 Classical Strain Engineering Nathan Crook and Hal Alper CHAPTER 2 Tracer-based Analysis of Metabolic Flux Networks Michael Dauner CHAPTER 3 Integration of ‘Omics' Data with Genome-scale Metabolic Models Stephen Van Dien, Priti Pharkya, and Robin Osterhout CHAPTER 4 Strain Improvement via Evolutionary Engineering Byoungjin Kim, Jing Du, and Huimin Zhao CHAPTER 5 Rapid Fermentation Process Development and Optimization Jun Sun and Lawrence Chew CHAPTER 6 The Clavulanic Acid Strain Improvement Program at DSM Anti-Infectives Bert Koekman and Marcus Hans CHAPTER 7 Metabolic Engineering of recombinant E. coli for the Production of 3-Hydroxypropionate Tanya Warnecke Lipscomb, Matthew L. Lipscomb, and Michael D. Lynch CHAPTER 8 Complex System Engineering: A Case Study for an Unsequenced Microalga Michael Guarnieri,Yat-Chen Chou, Bryon S. Donohoe, Eric Knoshaug, Lieve Laurens and Phillip T. Pienkos CHAPTER 9 Meiotic Recombination-based genome shuffling of Saccharomyces cerevisiae and Schefferomyces stiptis for increased inhibitor tolerance to lignocellulosic substrate toxicity Dominic Pinel and Vincent J. J. Martin