Basic and Applied Aspects (eBook)

Preface 6
Contents 8
Contributors 16
Purification of Hepatoblasts from Fetal Mouse Livers by Using a Temperature- Reversible Gelation Polymer and Their Application in Regenerative Medicine 32
Computer Aided Rational Design of Biochemical Systems 38
DNA Microarray in Nutrigenomics and Cancer Prevention Research: Elucidating Molecular Signatures of Cancer Preventive Phytochemicals 44
DNA Microarray Technology Evaluation of Effect of Dietary Intake of Crude Rice Glycosphingolipids in Mice 50
Integrated Pathway Analysis of Genome-Wide Expression Changes Associated with Serum- Free Suspension Adaptation of an Antibody- Producing Chinese Hamster Ovary ( CHO) Cell Line 57
Affordable Mab by Ensuring Low Cog 63
Evaluation of Neurotoxicity of Environmental Chemicals Using Neural Stem Cells and Neuroblastoma Cells 70
Integration of Endothelial Cell-Covered Hepatocyte Spheroids for Construction of Vascularized Liver Tissue 74
Characterization of Cell-Sized Vesicles Induced from Human Lymphoid Cell Lines 80
Dendrimer-Immobilized Culture Surface as a Tool to Promote Aggregate Formation of Anchorage- Dependent Cells 85
Advantage of Alagln as an Additive to Cell Culture Medium 92
Supplementation of Phosphatidylcholine Protects the Hybridoma Cells from Apoptosis Induced by SerumWithdrawal 99
Monoclonal Antibody Productivity of Hybridoma Cells Under Active Hypothermic Growth Condition 103
Efficient Selection of Cell Clones with Higher Productivity in the Production of Recombinant Human Monoclonal Antibodies 108
Continuous Cell Production from Three Dimensional Hematopoietic Microenvironment in Polyurethane Foam 114
A New Approach for Drug Discovery and Differentiation Study Using Cutting-Edge 3d Cell Culture System 120
Dimensional Cell Culture Dish for In Vivo-Like Cell Culture 128
Characterization of the Erythropoietin (EPO) Protein Production in the Recombinant Human Cultured Cells, in which the Exogenous EPO Gene was Introduced into Hypoxanthine Phosphoribosyl Transferase ( HPRT) Gene Locus 134
Development of Protein High Expression System by Using Fructose Metabolism 140
Controlling Fucosylation Levels of Antibodies with Osmolality during Cell Culture 145
Comparison of Immunoresponses Between Cecal Patch Cells and PeyerÌs Patch Cells Stimulated by Bacterial Components 150
Conjugate of ß-Lactoglobulin with Carboxymethyl Dextran Increased the Antigen- Specific IgG1 Production, While It Decreased IgE Production Compared with Native ß- Lactoglobulin 155
Anti-Inflammatory Action of Epigallocatechin- 3- Gallate at a Physiological Concentration through the 67- KDA Laminin Receptor in Lipopolysaccharide- Stimulated Raw264.7 Cells 160
Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production 166
Production of Human Monoclonal Antibodies Against FC Epsilon Receptor I Alpha by Combining In Vitro Immunization with Phage Display 172
Enhancement of Hepatocyte Function Through Heterotypic Cell- Cell Interactions Using E- Cadherin- Expressing NIH3T3 Cells 179
Magnetic Cell-Patterning for Tissue Engineering 184
Magnetic Force-Based Tissue Engineering of Skeletal Muscle for Bio- Actuator 190
Fabrication of Skeletal Muscle Tissue from C2C12 Myoblast Cell Towards the Use as Bio- Actuator 196
Retroviral Vectors Pseudotyped with Chimeric Vesicular Stomatitis Virus Glycoprotein for Antibody- Dependent Gene Transduction 203
Improvement of CPP-RBD for siRNA Delivery 209
Production of Recombinant Human EPO and EPO/ Fc Fusion Proteins by Chinese Hamster Ovary Cells 215
Development of Oviduct-Specific Gene Expression System for Transgenic Avian Bioreactor 221
Production of Therapeutic Proteins Composed of Seven Dominant Human T Cell Epitopes Derived from the Japanese Cedar Pollen Allergens 227
The Differentiation of C2C12 Cells to Myotube by Paraquat 233
Molecular Cloning and Characterization of a Novel Canine Sulfotransferase 238
In Vitro Analysis of the Interaction Between Human Intestinal Epithelial Cells and Macrophage- Like Cells 247
Role of Ubiquitination Promoted During Restimulation in the Induction of T CellAnergy 253
Expression Analysis of Senescence-Associated Genes: Their Possible Involvement in Diabetes 258
Study of Butyrate Signal Transduction Pathways in Rat Hepatic Stem- Like Cells 262
Growth Control of Mammalian Cells via a Human Artificial Chromosome Harboring a Chimeric Receptor 268
The Impact of Redox State on Regulation of the High- Affinity IgE Receptor Expression 274
The Characteristic of Chimeric Receptors Based on Erythropoietin Receptor 280
Anti-Cancer and Structure-Activity Relationship of Natural Polyacetylenes 286
Effect of Mongolian Medicinal Plant Stellera Chamaejasme on Chronic Leukemia Cells K562 291
Capsaicin Induced the Upregulation of Transcriptional and Translational Expression of Glycolytic Enzymes Related to Energy Metabolism in Human Intestinal Epithelial Cells 295
Effect of Novel Compounds from Thymelaea Hirsuta on Melanogenesis 298
Electrolyzed Reduced Water Prolongs Caenorhabditis elegans' Lifespan 301
Anti-Cancer Effects of Enzyme-Digested Fucoidan Extract from Seaweed Mozuku 306
The Downregulation of Mast Cell Activation Through the Suppression of the High- Affinity IgE Receptor Expression by Green Tea Catechin Egcg 312
Anti-Diabetes Effects of Hita Tenryou-Sui WaterR , a Natural Reduced Water 318
Suppressive Effect of ERW on Lipid Peroxidation and Plasma Triglyceride Level 325
Growth Suppression of HL60 and L6 Cells by Atomic Hydrogen 332
Insulin-Mimetic Activity of Inositol Derivatives Depends on Phosphorylation of PKC in L6 Myotubes 335
Enhancement of Phagocytic Activity by a Crude Polysaccharide from Tea ( Camellia sinensis) Extract 340
Effects of Lupeol on Melanoma In Vitro and In Vivo: Fundamental and Clinical Trials 346
The Effect of Secoisolariciresinol on 3t3-L1 Adipocytes and the Relationship Between Molecular Structure and the Activity 352
Detection of Anti-Allergic Factors in Strawberry Extracts 359
Anti-Melanogenic Activity of Yacon Leaves in Mouse Melanoma Cells 364
Cytokine Responses of Splenocytes of Female and Male Non- Obese Diabetic Mice Induced by Lactic Acid Bacteria 370
Development of a High-Throughput Evaluation System for Antiviral Activities of Food Factors By Using Bioinformatics 376
The Effects of Pesticides on Immune Cells 382
Co-Cultured Hepatocyte-Spheroid Chip Constructed Using the Microfabrication Technique 388
Micropatterned Hepatocyte Culture Using Microstencil Technique 395
Structural Features of Totally Designed Lipidic Materials for Small Interfering RNA Delivery System 401
Thin-Film Assembly of Totally Designed Lipidic Materials as Gene Carrier in Mouse Embryonic Neural Stem Cells: Thin- Film Assembly of Lipidic Material as Potential Gene Carrier 407
Transfection System Using a Cocktail of Totally Designed Lipidic Materials in Chinese Hamster Ovary Cells: Lipidic Cocktail as Potential Gene Carrier for CHO Cells 413
Size Dependent Heat Generation of Magnetic Nanoparticles Under AC Magnetic Field for Cancer Therapy 419
Functional Evaluation of Anticancer Drugs in Human Leukemia Cells Based on Metabolic Profiling Technique 426
Extracts from the Root of Platycodon grandiflorum Modulate Induction of Inflammatory Changes in Coculture of Adipocytes and Macrophages 432
Position Specific Incorporation of Nonnatural Amino Acids into Proteins In Vivo 438
Novel Protease Inhibitor Inhibiting Proliferation of Influenza Virus: Purification and cDNA Cloning of a Novel Protease Inhibitor Secreted by MDCK Cells 443