Neural Stem Cells

Definitions and Criteria for Stem Cells.- Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard.- Isolation and Culture of NSCs.- Neural Differentiation of Human Embryonic Stem Cells.- Generating Neurons from Stem Cells.- Isolation of Neural Stem and Precursor Cells from Rodent Tissue.- Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization.- Adult Neural Stem Cells.- Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb.- Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium.- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property.- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood.- Production of Neurospheres from CNS Tissue.- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells.- In Vitro Studies: A. Cellular Techniques.- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures.- Telomere Neurobiology.- Chromosomal Mosaicism in Neural Stem Cells.- In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype.- In Vitro Migration Assays of Neural Stem Cells.- Measuring Apoptosis in Neural Stem Cells.- Markers of Adult Neural Stem Cells.- In Vitro Studies: B. Molecular Techniques.- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR.- Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells.- Electroporation Strategies for Genetic Manipulation and Cell Labeling.- Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches.- Studies for Transplantation.- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo.- Immunocytochemical Analysis of Neuronal Differentiation.- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold.- Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS.- Optimizing Stem Cell Grafting into the CNS.- Routes of Stem Cell Administration in the Adult Rodent.