Mechanobiology

Measuring Cell Mechanical Properties Using Microindentation.- In Situ Measurements of Cell Mechanical Properties Using Force-Spectroscopy.- Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using Atomic Force Microscopy-Based Force Spectroscopy.- Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single Molecule Atomic Force Microscopy.- Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber.- Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretching.- Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo.- A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity-Sensing by Plant’s Roots and Root Hair Cells.- Photoresponsive Hydrogels for Studying Mechanotransduction of Cells.- Analyzing Mechanically Evoked Currents at Cell-Substrate Junctions.- Quantifying Strain Sensing Protein Recruitment During Stress Fiber Repair.- Quantification of Invadopodia Formation and Matrix Degradation Activity.- Measuring Cellular Traction Forces with Micropillar Arrays.- Imaging Cell Adhesive Force at the Single Molecule Level.- Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM.- Visualizing Neurons under Tension In Vivo with Optogenetic Molecular Force Sensors.- Single-Cell Quantification of the Mechanical Stability of Cell-Cell Adherens Junction Using Glass Micropipettes.- Using Micropatterned Supported Lipid Bilayers to Probe the Mechanosensitivity of Signaling Receptors.- Monitoring Mechano-Regulation of Gene Expression by RNA Sequencing.- Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK Phosphorylation.- Long-Term Fluorescence Recovery After Photobleaching (FRAP).- Simulating 3D Cell Shape with the Cellular Potts Model.