Epigenetics Protocols

Buch | Hardcover

304 Seiten

2004
Humana Press Inc. (Verlag)
978-1-58829-336-7 (ISBN)

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The field of epigenetics has grown exponentially in the past decade, and a steady flow of exciting discoveries in this area has served to move it to the forefront of molecular biology. Although epigenetics may previously have been considered a peripheral science, recent advances have shown considerable progress in unraveling the many mysteries of nontraditional genetic processes. Given the fast pace of epigenetic discoveries and the groundbreaking nature of these developments, a thorough treatment of the methods in the area seems timely and appropriate and is the goal of Epigenetics Protocols. The scope of epigenetics is vast, and an exhaustive analysis of all of the techniques employed by investigators would be unrealistic. However, this ™ volume of Methods in Molecular Biology covers three main areas that should be of greatest interest to epigenetics investigators: (1) techniques related to analysis of chromatin remodeling, such as histone acetylation and methylation; (2) methods in newly developed and especially promising areas of epigenetics such as telomere position effects, quantitative epigenetics, and ADP ribosylation; and (3) an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure.

Methods of Epigenetic Analysis.- Chromatin Immunoprecipitation Assays.- Native Chromatin Immunoprecipitation.- Q-PCR in Combination With ChIP Assays to Detect Changes in Chromatin Acetylation.- Restriction Endonuclease Accessibility as a Determinant of Altered Chromatin Structure.- Measuring Changes in Chromatin Using Micrococcal Nuclease.- DNaseI Hypersensitivity Analysis of Chromatin Structure.- Inhibition of Histone Deacetylases.- Site-Specific Analysis of Histone Methylation and Acetylation.- Analysis of Mammalian Telomere Position Effect.- Activity Assays for Poly-ADP Ribose Polymerase.- Multigenerational Selection and Detection of Altered Histone Acetylation and Methylation Patterns.- Profiling DNA Methylation by Bisulfite Genomic Sequencing.- Methylation-Sensitive Single-Strand Conformation Analysis.- SIRPH Analysis.- Real-Time PCR-Based Assay for Quantitative Determination of Methylation Status.- Denaturing Gradient Gel Electrophoresis to Detect Methylation Changes in DNA.- Photocrosslinking Oligonucleotide Hybridization Assay for Concurrent Gene Dosage and CpG Methylation Analysis.- Methylation-Specific Oligonucleotide Microarray.- Methylation-Specific PCR In Situ Hybridization.- Relative Quantitation of DNA Methyltransferase mRNA by Real-Time RT-PCR Assay.- DMB (DNMT-Magnetic Beads) Assay.

Erscheint lt. Verlag	23.7.2004
Reihe/Serie	Methods in Molecular Biology ; 287
Zusatzinfo	XII, 304 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	1-58829-336-X / 158829336X
ISBN-13	978-1-58829-336-7 / 9781588293367
Zustand	Neuware