Protein Phosphatase Protocols

Analysis of Protein Phosphatases.- A Brief Introduction to the Protein Phosphatase Families.- Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases.- Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose.- Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated.- An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity.- An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species.- Mutagenesis and Expression of the Scaffolding A? and A? Subunits of PP2A.- Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits.- Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies.- Purification of PP2Ac from Bovine Heart.- Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions.- Yeast Two-Hybrid Screens to Identify Drosophila PP1-Binding Proteins.- Identification of Cellular Protein Phosphatase-1 Regulators.- Assay for Three-Way Interaction of Protein Phosphatase-1 (Glc7) With Regulatory Subunits Plus Phosphatase Inhibitor-2.- Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C.- Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin.- Proteins Interacting With Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry.- Expression of Protein Histidine Phosphatase in Escherichia coli, Purification, and Determination of Enzyme Activity.- The Use of RNA Interference to Analyze Protein Phosphatase Function in MammalianCells.- Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases.- Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases.- Targeting of PP2C in Budding Yeast.- Phosphatase Targets in TOR Signaling.- Functional Characterization of Small CTD Phosphatases.- Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences.- Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases.