Golden Gate Cloning

Golden Gate Cloning of MoClo Standard Parts.- Assembly of Multigene Constructs using the Modular Cloning System MoClo.- Selection of Fusion-Site Overhang Sets for High Fidelity and High Complexity Golden Gate Assembly.- Modular DNA Construct Design for High-Throughput Golden Gate Assembly.- Using ApE for In Silico Golden Gate Cloning.- Standardized Golden Gate Assembly Metadata Representation using SBOL.- Use of a Golden Gate Plasmid Set Enabling Scarless MoClo-Compatible Transcription Unit Assembly.- Biofoundry-Assisted Golden Gate Cloning with AssemblyTron.- Automation and Miniaturization of Golden Gate DNA Assembly Reactions using Acoustic Dispensers.- Validation of Golden Gate Assemblies using Highly Multiplexed Nanopore Amplicon Sequencing.- Modular Golden Gate Assembly of Linear DNA Templates for Cell-Free Prototyping.- Construction of Small Genes with Repetitive Elements using Oligo Extension and Golden Gate Assembly.- Simplifying Recombinant Protein Production: Combining Golden Gate Cloning with a Standardized Protein Purification Scheme.- Generating Site Saturation Mutagenesis Libraries and Transferring Them to Broad Host-Range Plasmids using Golden Gate Cloning.- Combining Oligo Pools and Golden Gate Cloning to Create Protein Variant Libraries or Guide RNA Libraries for CRISPR Applications.- Golden Gate Cloning of Synthetic CRISPR RNA Spacer Sequences.- Golden Gate Cloning of Expression Plasmids for Synthetic Small RNAs in Bacteria.- Utilizing Golden Gate Assembly to Streamline CRISPR-Cas/NgTET-Based Phage Mutagenesis.- Golden Gate Assisted Gene Doctoring for Streamlined and Efficient Recombineering in Bacteria.- Protocol for NT-CRISPR, A Method for Efficient Genome Engineering in Vibrio natriegens.- Golden Gate Cloning in Actinobacteria: Opportunities and Challenges.- Golden Gate Assembly of Transcriptional Unit Libraries into a Rearrangeable Gene Cluster.- Golden Gate Cloning for Efficient Biosynthesis of Lycopene in Synthetic Yeast.- YeastFab Cloning of Toxic Genes and Protein Expression Optimization in Yeast.- Golden Gate Cloning for the Standardized Assembly of Gene Elements with Modular Cloning in Chlamydomonas.- Assembling DNA Plasmids with the Multi-Kingdom (MK) Cloning System.- Advancements in Golden Gate Cloning: A Comprehensive Review.