TRNA-derived RNAs

1. Purification of endogenous tDRs by hybridization-based pulldown
Yasutoshi Akiyama
2. Northern blotting for human pre-tRNA and tRNA-derived RNAs
Yasutoshi Akiyama
3. In vitro translation systems to study tDRs
Pavel Ivanov
4. tRNA derived RNAs that form tetramolecular assemblies
Prakash Kharel
5. Ribozyme-mediated expression of tRNA-derived small RNAs in bacteria
Norbert Polacek
6. Ribosome-associated tDRs in yeast
Norbert Polacek
7. tDR bioinformatics
Todd Lowe
8. cP-RNA-seq for the specific sequencing of tRNA halves and other cP-containing RNAs
Yohei Kirino
9. TaqMan RT-qPCR for specific quantification of tRNA halves and other small RNAs
Yohei Kirino
10. Detection and isolation extracellular tDRs
Alfonso Cayota
11. Preparation of nicked tRNAs
Juan Pablo Tosar and Alfonso Cayota
12. In vitro functional analysis of plant tDRs
Laurence Drouard
13. TGIRT-seq to profile tRNA-derived RNAs and associated RNA modifications
Anindya Dutta, Zhangli Su, Abigail Johnston and Monima Anam
14. A general framework to over-express tRNA-derived RNAs from their parental tRNAs in mammalian cells
Anindya Dutta, Zhangli Su, Kumarjeet Banerjee and Fatemeh Esmaeili
15. Functional characterization of tRNA-derived small RNAs in stem cells
Cristian Bellodi
16. Detection of mitochondrial tDRs in killifish
Claire Riggs, Gazal Kalyan and Jason Podrabsky
17. Measurement and comparison of full-length tRNA and tRF abundance and modifications
Tao Pan and Luke Frietze
18. Using a Fluorescence based tRNA to image tDR biogenesis and localization.
Saumya Das and Guoping Li
19. Manipulation of tDR expression using gain of function and loss of function approaches
Saumya Das and Guoping Li
20. TBD
Mark A. Kay and Hak Kyun Kim
21. Nicked tRNAs in bacteria
Sandra Wolin
22. Determining Small RNA-Interacting Proteomes Using Endogenously Modified tRNA-Derived RNAs
Matthias Ronald Schaefer, Vera Oberbauser and Aleksej Drino
23. Activation of angiogenin by ribosome to produce tRNA-derived RNAs
Andrei A. Korostelev and Anna B. Loveland