Transmembrane β-Barrel Proteins

The Name is Barrel, β-Barrel.- Recombinant Expression and Overproduction of Transmembrane b-Barrel Proteins.- Bacterial Envelope Fractionation.- Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System.- In Vitro Reconstruction of Bacterial β-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane.- Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes.- In Vivo Disulfide-Bond Crosslinking to Study β-Barrel Membrane Protein Interactions, Dynamicity, and Folding Intermediates.- Site-Specific Photocrosslinking to Investigate Toxin Delivery Mediated by the Bacterial β-Barrel Assembly Machine.- Analysis of Transmembrane β-Barrel Proteins by Native and Semi-Native Polyacrylamide Gel Electrophoresis.- Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs.- Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery.- MitoLuc:A Luminescence-Based Assay to Study Real-Time Protein Import into Mitochondria.- Modular Assembly of Mitochondrial β-Barrel Proteins.- Tracking the Activity and Position of Mitochondrial β-Barrel Proteins.- Conformational Heterogeneity of β-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy.- Characterization of β-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry.- Dissecting the Organization of a β-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry.- Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel.- Recent Advances in Modeling Membrane β-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies.- Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes.- Rationale in Custom Design of Transmembrane β-Barrel Pores.- Stress-Based Screening for Compounds that Inhibit β-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria.