Metagenomics

Construction of Small-Insert and Large-Insert Metagenomic Libraries.- Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies.- Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds.- Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA.- Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next Generation Sequencing Approach.- Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes.- Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity.- Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities.- Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures.- The PET-Degrading Potential of Global Metagenomes: From InSilico Mining to Active Enzymes.- High-Throughput Screening for Thermostable Polyester Hydrolases.- Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System.- Assigning Functions of Unknown Enzymes by High Throughput Enzyme Characterization.- Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incapable of Plaque Formation.- CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis.- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production.- DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms.