Melatonin

Two-Dimensional Thin Layer Chromatography of Melatonin and Related Compounds.- Detection of Melatonin in Tissue Samples.- Measuring Dim Light Melatonin Onset in Humans.- Measuring Urinary 6-sulphatoxymelatonin in Humans.- MEL-Index: Estimation of Tissue Melatonin Levels using Gene Expression Data.- Melatonin Synthesis Enzymes Activity: Radiometric Assays for AANAT, ASMT, and TPH.- Pinealectomy in Rats.- Surgical Techniques and Nuances for Superior Cervical Ganglionectomy and Decentralization in Rats.- Pineal Microdialysis.- Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland.- Pineal Cells Dissociation and Culture: Isolated Pinealocytes, Isolated Astrocytes, and Co-Culture.- Pineal Gland Culture.- Preparation of Single Pineal Cells.- RNA Sequencing of Single Pineal Cells.- siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells.- Analysis of the Human Pineal Proteome by Mass Spectrometry.- The Pineal Transcriptome Webpage.- 2-[125I]iodomelatonin and [3H]melatonin Binding Assays for Melatonin Receptors.- Alternative Ligands at Melatonin Receptors.- GTPS Binding Assay for Melatonin Receptors in Mouse Brain Tissue.- MT1 Melatonin Receptor Reconstitution in Nanodiscs.- Functional Investigation of Melatonin Receptors Activation by Homogenous cAMP Assay.- Functionality of Melatonin Receptors: Internalization.- Functionality of Melatonin Receptors: Recruitment of β-arrestin at MT1.- MT1 Receptor Signaling Pathways by Impedance Measurement.- Measuring Protein-Protein Interactions of Melatonin Receptors by Bioluminescence Resonance Energy Transfer (BRET).- Non-Radioactive In Situ Hybridization of MT1 Melatonin Receptor for the Identification of Melatonin Target Cells.- Beta-Galactosidase as a Transgenic Reporter for the Mapping and Phenotyping of MT1 and MT2 Melatonin Receptor Expressing Cells.- Why Are We Still Cloning Melatonin Receptors? A Commentary.- Measuring Binding at the Putative Melatonin Receptor MT3.- Cloning, Expression, Purification, Crystallization, and X-Ray Structural Determination of the Human NQO2 in Complex with Melatonin.- Melatonin Binding to Human NQO2 by Isothermal Titration Calorimetry.- Measurement of NQO2 Catalytic Activity and of Its Inhibition by Melatonin.- Measuring the NQO2: Melatonin Complex by Native Nano-Electrospray Ionization Mass Spectrometry.- Human and Rodent Cell Lines as Models of Functional Melatonin-Responsive Pancreatic Islet Cells.- Mesenchymal Stem Cell and Monocyte Co-Cultures.- Real-Time Monitoring of Circadian Rhythms in the Eye.- Assessing the Role of Melatonin in the Modulation of Visual Functions in the Mouse.- Methods to Assess Melatonin-Receptor Mediated Phase-Shift and Re-Entrainment of Rhythmic Behaviors in Mouse Models.- Neurotrasmission Recovery by Melatonin Measured by CMAP.- Olfaction and Melatonin: The Use of the Olfactory Discrimination Test.- Photothrombotic Mouse Models for the Study of Melatonin as a Therapeutic Tool After Ischemic Stroke.- Hypnotic Effects of Melatonergic Compounds Measured in Mice or Rats.- Acute and Chronic Pain Preclinical Models to Study the Analgesic Properties of Melatonergic Compounds.- Behavioral and Fluorescent-Based Immunohistochemistry Protocols for Examining Anti-Depressant-Like Effects of Melatonin in Mice.- A Method for Perfusion of Tissue-Isolated Human Tumor Xenografts in Nude Rats to Investigate the Oncostatic Role of the Physiological Nocturnal Melatonin Signal.- A Method for Growing Tissue-Isolated Human Tumor Xenografts in Nude Rats for Melatonin/Cancer Studies.