Plant Tissue Culture Manual - Supplement 5

Section A Basic Techniques: Cell and Tissue Culture of Model Species. 1. Media preparation; O.L. Gamborg. 2. The initiation and maintenance of callus cultures of carrot and tobacco; R.D. Hall. 3. The initiation and maintenance of plant cell suspension cultures; R.D. Hall. 4. Shoot cultures and root cultures of tobacco; J.F. Topping, K. Lindsey. 5. Somatic embryogenesis in orchardgrass; M.E. Horn. 6. Arabidopsis regeneration and treasformation (leaf and cotyledon explant system); R. Schmidt, L. Willmitzer. 7. Arabidopsis protoplast transformation and regeneration; B. Damm, L. Willmitzer. 8. Arabidopsis regeneration and transformation (root explant system); D. Valvekens, M. van Lijsebettens, M. van Montagu. 9. Somatic embryogenesis in carrot; A.D. Krikorian, D.L. Smith. 10. Low density cultures: microdroplets and single-cell nurse cultures; G. Spangenberg, H.-U. Koop. 11. Tobacco protoplast isolation, culture and regeneration; I. Negrutiu. Section B Tissue Culture and Transformation of Crop Species. 1. Embryogenic callus, cell suspension and protoplast cultures of cereals; I.K. Vasil, V. Vasil. 2. Transformation and regeneration of rice protoplasts; J. Kyozuka, K. Shimamoto. 3. Transformation and regeneration of orchardgrass protoplasts; M.E. Horn. 4. Transformation and regeneration of oilseed rape protoplasts; D. Rouan, P. Guerche. 5. Regeneration and transformation of potato by Agrobacterium tumefaciens; R.G.F. Visser. 6. Transformation of tomato with Agrobacterium tumefaciens; S. McCormick. 7. Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens; K. Lindsey, P. Gallois, C. Eady. 8. Regeneration and transformation of apple (Malus pumila Mill.); D.J. James, A.M. Dandekar. 9. Transformation and regeneration of maize protoplasts; C.A. Rhodes, D.W. Gray. 10. Regeneration and transformation of barley protoplasts; P. Lazzeri, A. Jahne, H. Lorz. 11. Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation; J.P. Spychalla, M.W. Bevan. Section C Propagation and Conservation of Germplasm. 1. Clonal propagation of orchids; Y. Sagawa. 2. Clonal propagation of palms; B. Tisserat. 3. Clonal propagation of conifers; T.A. Thorpe, I.S. Harry. 4. Cytological techniques; A. Karp. 5. Restriction fragment analysis of somaclones; R.H. Potter. 6. Virus elimination and testing; M.C. Coleman, W. Powell. 7. Clonal propagation of Citrus; T.S. Rangan. 8. Clonal propagation of Eucalypts; J.A. McComb. Section D Direct Gene Transfer and Protoplast Fusion. 1. Gene transfer by particle bombardment; T.M. Klein, S. Knowlton, R. Arentzen. 2. Transformation of pollen by particle bombardment; D. Twell, T.M. Klein, S. McCormick. 3. Electrical fusion of protoplasts; M.G.K. Jones. 4. Cybrid production and selection; E. Galun, D. Aviv. (Part contents).