Confocal Microscopy for Biologists

1. What Is Confocal Microscopy?.- 2. Understanding Microscopy.- 3. Confocal Microscopy Hardware.- 4. Image Collection.- 5. Digital Images In Microscopy.- 6. Imaging Software.- 7. Presentation And Publication.- 8. What Is Fluorescence?.- 9. Fluorescent Probes.- 10. Confocal Microscopy Techniques.- 11. Fluorescence Immunolabelling...- 12. Imaging Live Cells.- 13. The Internet.- 14. Technical Supplies.- 15. Further Reading.- Appendix 1: Confocal Microscopes.- Laser Spot Scanning Confocal Microscopes.- Nipkow Spinning Disk Confocal Microscopes.- Nipkow Disk / Micro-lens Array Confocal Microscopes.- A. Bio-Rad Cell Science Division.- Bio-Rad Instruments.- Radiance Series of Confocal Microscopes.- Components of the Radiance Confocal Microscopes.- Radiance Scan Head.- Radiance Instrument Control Unit (ICU).- Changing Optical Filters and Dichroic mirrors.- Radiance 2100 Rainbow.- Sels (Signal Enhancing Lens).- Photon Counting.- Computer and Software.- Mrc Series of Confocal Microscopes.- Components of the Mrc-1024 Confocal Microscope.- Mrc-1024 Scan Head.- Scan Head Alignment.- Optical Filter Blocks.- Emission Filter Wheels.- Photomultiplier Tubes (PMT).- Controller Box.- Computer Requirements.- Hardware set up for specific imaging methods.- Dual and Triple labelling — T 1 and T2a Filter Blocks.- Live Cell Work — B1 and Open Block Filter Blocks.- Back Scatter Imaging — B1 and T 1 Filter Blocks.- Trouble Shooting (Mrc And Radiance).- Bio-Rad Lasersharp Software.- Image Collection Panel.- Main Control Panel.- Optics Control Panel.- Mixer Control Panel.- Carl Zeiss Microscopy.- Zeiss Confocal Microscopes.- Zeiss Lsm 510 Meta Confocal Microscope.- Components of the Zeiss 510 Meta.- Zeiss Lsm 510 Meta Scan Head.- Lasers.- Single Track and Multi Track Image Collection.-Conventional Detection Channels.- Meta Channel.- Transmission Imaging.- Zeiss Confocal Microscope Control Software.- Main Control Panel.- Setting un the Microscope and Imaging Channels.- vSetting the Scan Control Parameters.- Adjusting Imagine Channel Settings.- Image Collection Panel.- Using the Meta Channel.- Meta Channel as a multi-Band Pass Optical Filter.- Meta Channel — Lambda Mode (Spectral Imaging).- Extracting Image Channels from a Spectral Stack.- Emission Fingerprinting.- On-Line Emission Fingerprinting.- Leica Microsystems.- Leica Confocal Microscopes.- Components of the Leica Spectral Imaging Instrument.- Leica Tcs Sp2 Confocal Microscope.- Leica “Ics Sp2 Scan Head.- Acoustic Optical Beam Splitter (AOBS).- Spectral Separation.- Uv Excitation.- Multi-Photon Excitation.- Transmission (DIC) Imaging.- Leica Confocal Microscope Control Software.- Main Control Panel — Monitor 1.- Changing the Desktop Knob Functions..- “Beam Path Setting” Control Panel.- Control “Buttons” for Image Collection.- Image Collection Panel — Monitor 2.- Nikon Instruments.- Nikon Confocal Microscopes.- Components of the Nikon C 1 Confocal Microscope.- Nikon C1 Scan Head.- Optics/Detector Unit.- Laser Launch Unit.- Nikon C1 User Interface.- Olympus Corporation.- Olympus Confocal Microscopes.- Components of the Olympus Fluoview 1000.- Fluoview 1000 Confocal Microscope.- Sim (Simultaneous) Scanner.- Fluoview 500 Confocal Microscope.- Fluoview 500 Scan Head.- Components of the Olympus Fluoview 300 and 500.- Fluoview 300 Confocal Microscope.- Fluoview 300 Scan Head.- Fluoview Software.- Image Collection Panel.- Main Control Panel.- Instrument settings Tabs.- Atto Bioscience.- Atto Bioscience Confocal Microscopes.- Carv Confocal Microscopes.- Components of the Carv ContocalMicroscope.- Carv Scan Head.- Yokogawa Electric Corporation.- CSU10 Scan Head.- CSU21 Scan Head.- Perkinelmer Life Sciences.- Perkinelmer Confocal Microscopes.- Ultra View Lc1 layout.- Components of the PerkinElmer Ultra View.- Visitech International.- Visitech Confocal Microscopes.