The Nuclear Pore Complex

Affinity Isolation of Endogenous Saccharomyces cerevisiae Nuclear Pore Complexes.- Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes.- Nuclear Pore Complex Assembly using Xenopus Egg Extract.- Analysis of Nuclear Pore Complex Permeability in Mammalian Cells and Isolated Nuclei Using Fluorescent Dextrans.- Hormone-inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases.- Subcellular Fractionation Suitable for Studies of RNA and Protein Trafficking.- Localising Total mRNA in Plant Cells.- Using Single Molecule RNA FISH to Determine Nuclear Export and Transcription Phenotypes in Drosophila Tissues.- Analysis of Nucleoporin Function using Inducible Degron Techniques.- Monitoring of Chromatin Organization at the Nuclear Pore Complex, Inner Nuclear Membrane and Nuclear Interior in Live Cells by Fluorescence Ratiometric Imaging of Chromatin (FRIC).- Analysis of Nuclear Pore Complexes in Caenorhabditis elegans by Live Imaging and Functional Genomic.- Protein Retargeting in Aspergillus nidulans to Study the Function of Nuclear Pore Complex Proteins.- Split-GFP Complementation to Study the Nuclear Membrane Proteome using Microscopy.- Bimolecular Fluorescence Complementation: Quantitative Analysis of In Cell Interaction of Nuclear Transporter Importin a with Cargo Protein.- Validation of Nuclear Pore Complex Protein-Protein Interactions by Transient Expression in Plants.- Binding affinity measurement of Nuclear Export Signal peptides to their exporter CRM1.- Analysis of Ubiquitylation and SUMOylation of Yeast Nuclear Pore Complex Proteins.- Purification of Cdk-CyclinB-Kinase-Targeted Phosphopeptides from Nuclear Envelope.- Crystallization of Nuclear Export Signals or Small Molecule Inhibitors Bound to Nuclear Exporter CRM1.- Atomic Force Microscopy for Structural and Biophysical Investigations on Nuclear Pore Complexes.- Multivalent Interactions with Intrinsically DisorderedProteins Measured by Surface Plasmon Resonance.- Assembly and Use of a Microfluidic Device to Study Nuclear Mechanobiology during Confined Migration.- SPEED Microscopy: High-Speed Single-Molecule Tracking and Mapping of Nucleocytoplasmic Transport.- Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans.- Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts.- TEM Imaging of Membrane Choreography During Mitosis of Drosophila Tissue Culture Cells.- Scanning Electron Microscopy (SEM) and Immuno-SEM of Nuclear Pore Complexes from Amphibian Oocytes, Mammalian Cell Cultures, Yeast and Plants.- NPC Structure in Model Organisms: Transmission Electron Microscopy and Immuno-gold Labelling using High Pressure Freezing/Freeze Substitution of Yeast, Worms and Plants.- High Resolution Imaging and Analysis of Individual Nuclear Pore Complexes.- Live CLEM imaging of Tetrahymena to Analyze the Dynamic Behavior of the Nuclear Pore Complex.- Visualizing Nuclear Pore Complex Assembly In Situ in Human Cells at Nanometer Resolution by Correlating Live Imaging with Electron Microscopy.