Celiac Disease

Ainara Castellanos-Rubio obtained her PhD on Genetics from the University of the Basque Country (Spain) in 2010. During her PhD, she studied gene expression alterations and genetic polymorphisms associated to Celiac Disease. She did a short term research stay in the University of Tampere (Finland) under the supervision of Dr. Marku Makki and Dr. Katri Lindfors where she used three dimensional cell cultures to describe different pathways involved in Celiac Disease development. On 2011 she joined the Laboratory of Dr. Sankar Ghosh in Columbia University (NY, USA) where she carried out her postdoctoral studies. During her postdoctoral training, she studied the implication of long noncoding RNAs (lncRNAs) in the immune and inflammatory response and she discovered and functionally characterized a novel lncRNA involved in the susceptibility to Celiac Disease. Dr. Castellanos-Rubio is an Ikerbasque Associate in the University of the Basque Country, where she leads her own group. She is interested in the involvement of noncoding RNAs in the pathogenesis of autoimmune and inflammatory diseases and studies the influence of disease associated SNPs in the functional disturbance of these RNAs. More recently, she has become interested on the epitranscriptomic alterations involved in different aspects of RNA regulation and her group studies how SNPs and environmental factors can alter these epitranscriptomic signals influencing the inflammatory response that finally evolves in disease development. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. Pepsin-trypsin digested gliadin treatment in intestinal cells
Ane Olazagoitia-Garmendia

2. Ex vivo gliadin stimulation of intestinal cells
Linda Zhang and Chuan He

3. Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers
Olga Biskou and Amaia Jauregi-Miguel

4. Intestinal permeability assessment using lactulose and mannitol in celiac disease
Silvia Martinez Velasco, Azucena Gonzalez Garcia, Inaki Xarles Irastorza Terradillos, and
Jose Ramon Bilbao Catala

5. In vivo sensitization to gliadin by oral administration
M. Mar Romero, Dolors Serra, Ainara Castellanos-Rubio, and Laura Herrero

6. Separation of epithelial and immune cells from biopsy samples
Irati Romero-Garmendia

7. Study and isolation of human intestinal dendritic cell and macrophage subsets
Elisa Arribas-Rodriguez, Luis Fernandez-Salazar, Beatriz de Andres, Eduardo Arranz, Jose A. Garrote, and David Bernardo

8. Elucidating the role of microbes in celiac disease through gnotobiotic modeling
Alberto Caminero, Elena F. Verdu, and Heather J. Galipeau

9. In vitro differentiation of macrophages from peripheral blood cells of celiac patients
Federica Farina, Mariavittoria Laezza, Alessio Fasano, and Giovanna Del Pozzo

10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge
Concepcion Nuñez, Sara Gomez-Aguililla, Maria Corzo, F. Fernandez-Bañares, A. Bodas, S. Farrais,
N. Lopez-Palacios, and Mercedes Rubio

11. Isolation and cryopreservation of peripheral blood mononuclear cells
Sara Gomez-Aguililla, Concepcion Nuñez, Mercedes Rubio, and Maria Corzo

12. Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease
Concepcion Nuñez, A. Carrasco, Maria Corzo, R. Pariente, M. Esteve, and G. Roy

13. The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin
Francesco Maurano, Vera Rotondi Aufiero, Lucia Treppiccione, Stefano Rossi, Diomira Luongo, Giuseppe Mazzarella, and Mauro Rossi

14. Human intestinal organoid models for celiac disease research
Valeriia Dotsenko, Amir-Babak Sioofy-Khojine, Heikki Hyoty, and Keijo Viiri

15. Preparation of pepsin trypsin digested gliadin for stimulation experiments
Luis Manuel Mendoza-Gomez, Maialen Sebastian-delaCruz, Ane Olazagoitia-Garmendia, Itziar Gonzalez-Moro, Henar Rojas-Marquez, Jon Mentxaka, Izortze Santin, and Ainara Castellanos-Rubio

16. A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs
Maialen Sebastian-delaCruz and Ainara Castellanos-Rubio