Handbook of HPLC For the Separation of Amino Acids, Peptides, and Proteins

Dr. William Hancock is Bradstreet Chair in Bioanalytical Chemistry, Barnett Institute of Chemical and Biological Analysis, Northeastern University.

Introduction to the General Principles of HPLC Introduction to High Performance Liquid Chromotagraphy. The Structure of Microparticulate Silica. Pore Structure of Chemically Modified Silica. Commercially Available Columns. HLPC Instrumentation Requirements for Suitable Instrumentation. Example of a Commercially Available Instrument – Micromeritics. Example of a Commercially Available Instrument – Varian. Example of a Commercially Avaiable Instrument – Shumadzu. Mobile Phases Manipulation of the Ion-Pair Effect in HPLC. An Ion-Interaction Model. Phosphates as Mobile Phase Modifiers. Perfluoroalkanoic Acids as Mobile Phase Modifiers. Hydrochloric Acid as a Mobile Modifier. Detection Improvement by Use of UV-Absorbing Counter-Ions. Detection Methods Detection Methods Suitable for Monitoring the Separation of Amino Acids, Peptides, and Proteins. Post-Column Fluorometric Detection. Field Desorption Mass Spectrometry. Reversed-Phase Thin-Layer Chromatography (RC-TLC). Radioimmunoassay. Separation of Free Amino Acids Review of Separation Conditions. Aromatic Amino Acids in Tissues. m- and o- Tyrosine. Cross-Links of Collagen. Iodothyronines and Iodotyrosines. Thyronines. Products Formed by Thyroid Peroxidase. o-Pthaldialdehyde Derivative of Taurine. o- Pthaldialdehyde Derivative of Amino Acids in Cocoa Beans. Available Lysine Using the Dinitrophenyl Derivative. Dansyl Amino Acids. Lysinoalnine in Foods as Measured Via Dansyl Derivative. Dabsyl Amino Acids. Amino Acid Phenylthiohydantoins. Resolution of Amino Acids as Diastereomeric Derivatives Resolution of Amino Acids as Diastereomers. Application of Ligand-Exchange Chromotography to Separation of Amino Acids, Peptides, and Proteins Review of Ligand-Exchange Chromotography (LEC). Separations Where the Ligand Is Bound to a Normal-Phase Column. Separations Where Ligand Is Bound to a Reversed-Phased System. Resolution of α-Amino Acids and Dns- α-Amino Acids by HLPC with Mobile Phases Containing a Chiral Ligand.