Methods in Stem Cell Biology - Part A

Dr Ilio Vitale is an esteemed oncologist. He is a member of the University of Rome and associated to the Regina Elena National Cancer Institute based in Italy. Gwenola Manic received her Ph.D. from the University of Paris Sud/Paris XI (France) in 2012 studying the impact of the design of HIV-1-derived vectors and DNA repair machinery on lentiviral expression. At present she is working as a postdoctoral fellow at the Department of Biology at the Università degli Studi di Roma “Tor Vergata” in Rome (Italy) on the contribution of chromosomal instability in tumorigenesis and the therapeutic targeting of cancer stem cells. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. Orthotopic brain tumor models derived from glioblastoma stem-like cells
Katharina M. Eyme, Alessandro Sammarco, and Christian E. Badr
2. Identification and isolation of slow-cycling glioma stem cells
Liam Furst, Ryan J. Atkins, Marija Dinevska, Stanley S. Stylli, Niall M. Corcoran, Christopher M. Hovens, and Theo Mantamadiotis
3. Immunohistochemical analysis of the developing mouse cortex
Mohamad-Reza Aghanoori, Kaylan M.L. Burns, Maneesha Subha, Laura Williams, Michelle Hua, Farzaneh Nobakht, Taylor Krawec, and Guang Yang
4. Subcellular fractionation of brain tumor stem cells
Ahmad Sharanek, Laura Raco, Vahab D. Soleimani, and Arezu Jahani-Asl
5. microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting
Chiara Tordonato, Matteo Jacopo Marzi, Pier Paolo Di Fiore, and Francesco Nicassio
6 Genome-wide RNA interference screen in cancer stem cells
Guillaume Pinna, Marie Vandamme, Celia Rouault, Emmanuelle Charafe-Jauffret, and Christophe Ginestier
7. Clonal analysis of human clonogenic keratinocytes
Elena Enzo, Camilla Cattaneo, Federica Consiglio, Maria Pia Polito, Sergio Bondanza, and Michele De Luca
8. Immunofluorescence analysis of myogenic differentiation
Atsushi Asakura and Nobuaki Kikyo
9. Generating pancreatic beta-like cells from human pluripotent stem cells
Lillian Yuxian Lim, Carmen Ching, Dewei Kong, Shiao-Yng Chan, and Adrian Kee Keong Teo
10. In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering
Jose Ramon Pineda, Yurena Polo, Beatriz Pardo-Rodríguez, Jon Luzuriaga, Verónica Uribe-Etxebarria, Patricia García-Gallastegui, José Ramón Sarasua, Aitor Larrañaga and Gaskon Ibarretxe
11. Identification of progenitor cells and their progenies in adult Drosophila midgut
Zhen Jin, Minhua Che, and Rongwen Xi
12. Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications
Luciana M. Domínguez, Esteban J. Fiore, and Guillermo D. Mazzolini
13. Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells
Yiren Qin, Fuqiang Geng, and Duancheng Wen