DNAzymes

A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzyme.- Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzyme.- PCR Methods for the Generation of Catalytic ssDNA.- Design of a DNAzyme: Prediction of mRNA Regions Accessible to a DNAzyme.- Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymes.- Stability and Activity of the 10-23 DNAzyme under Molecular Crowding Conditions.- Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR Spectroscopy.- Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking Method.- Crystallization and Structural Determination of 8-17 DNAzyme.- Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and Function.- Molecular Modeling and Simulations of DNA and RNA: DNAzyme as a Model System.- Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer.- Chemical Dual End-Labeling of Large Ribozymes.- Spin Labeling of Long RNAs via Click Reaction and Enzymatic Ligation.- Preparation of Site-Specifically Spin-Labeled RNA by In Vitro Transcription using an Expanded Genetic Alphabet.- PELDOR Measurements on Nitroxide-Labelled Oligonucleotides.- Sensing Metal Ions with Phosphorothioate-Modified DNAzymes.- Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-quadruplex-Forming Oligonucleotide Using Click Chemistry.- Generation of RNA with 2’, 3’-cyclic Phosphates by Deoxyribozyme Cleavage in Frozen Solutions.