Post-Transcriptional Gene Regulation

Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression.- Predicting RNA Secondary Structure Using In Vitro and In Vivo Data.- RBPmap: A Tool for Mapping and Predicting the Binding Sites of RNA-Binding Proteins Considering the Motif Environment.- Analysis of mRNA Translation by Polysome Profiling.- Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling.- Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq.- Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems.- Studying RNP Composition with RIP.- PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites.- A Pipeline for Analyzing eCLIP and iCLIP Data with htseq-clip and DEWSeq.- Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH).- Identifying the Protein Interactomes of Target RNAs Using HyPR-MS.- Visualization and Quantification of Subcellular RNA Localization Using Single Molecule RNA Fluorescence In Situ Hybridization.- Single-Molecule RNA Imaging Using Mango II Arrays.- Genome-Wide Identification of Polyadenylation Dynamics with TED-Seq.- In Vivo RNA Structure Probing with DMS-MaPseq.- Transcriptome-Wide Profiling of RNA Stability.- High-Throughput Quantitation of Yeast uORF Regulatory Impacts Using FACS-uORF.- m6A RNA Immunoprecipitation Followed by High-Throughput Sequencing to Map N6-Methyladenosine.- Detecting m6A with In Vitro DART-Seq.- Target-Specific Profiling of RNA m5C Methylation Level Using Amplicon Sequencing.- Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.