Cell Cycle Checkpoint Control Protocols

Induction and Detection of Changes in cell Cycle Progression.- Methods to Induce Cell Cycle Checkpoints.- Methods for Synchronizing Mammalian Cells.- Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation.- Analysis of the Mammalian Cell Cycle by Flow Cytometry.- Methods for Detecting Cells in S Phase.- Yeast Cell Synchronization.- Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow Cytometry.- Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle.- Analysis of Genes Involved in Checkpoint Control.- Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes.- Microarray Approaches for Analysis of Cell Cycle Regulatory Genes.- Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens.- Gene Targeting in Cultured Human Cells.- Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene.- In Vitro Mutagenesis to Define Functional Domains.- Use of Gene Overexpression to Assess Function in Cell Cycle Control.- Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression.- Analysis of Proteins Involved in Checkpoint Control.- Cataloging Proteins in Cell Cycle Control.- Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control.- Purification and Identification of Protein Complexes That Control the Cell Cycle.- Xenopus Cell-Free Extracts to Study DNA Damage Checkpoints.- Protein-Protein Interactions.- Detection of Kinase and Phosphatase Activities.- Monitoring Changes in the Subcellular Location of Proteins in S. cerevisiae.- Chromosomes and the Cell Cycle.- Chromosomal Changes and Cell Cycle Checkpoints in Mammalian Cells.- Detecting the Influence of Cell Cycle Regulatory Proteins on Human Telomeres.- Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence.

"...includes step-by-step instructions written by an investigator who performs it frequently, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls." - Tumori