Ion Channels: Channel Production and Optical Methods

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Preface

Daniel L. Minor Jr. and Henry M. Colecraft

Methods to produce and purify ion channels

1. Effective production of oligomeric membrane proteins by EarlyBac

Hiro Furukawa

2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies

Lejla Zubcevic

3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies

Vera Moiseekova-Bell

4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies

Seok-Yong Lee

5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination

William A. Catterall

6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy

Nieng Yan

7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy

Show-Ling Shyng

8. Production of K2P2.1 (TREK-1) for structural studies

Daniel L. Minor Jr.

9. Purification of a native nicotinic receptor

Ryan Hibbs

10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs

Stephen Brohawn

Optical methods for probing and controlling channel function

11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid

Sebastian E. Brauchi

12. Fluorescence lifetime measurement of absolute membrane potential

Evan Miller

13. Fluorescent Toxins as Ion Channel Activity Sensors

Jon Sack

14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer

Manu Ben-Johny

15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers

Thomas Grutter

16. Counting Subunits in Kv Channel Complexes

Rikard Blunck