Host-Fungal Interactions

Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans.- Serological Proteome Analysis for the Characterisation of Secreted Fungal Protein Antigens. Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification.- CRISPR-Cas9 Mediated Gene Silencing in Cultured Human Epithelia.- Analysis of Epithelial Cell Responses to Microbial Pathogens.- Single-cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluorescent Staining and Imaging Flow Cytometry.- Fungal Bioreporters to Monitor Outcomes of Blastomyces – Host Cell Interactions.- Fungal Bioreporters to Monitor Outcomes of Aspergillus – Host Cell Interactions.- Candida albicans Interaction with Oral Epithelial Cells: Adhesion, Invasion, and Damage Assays.- Experimental Evolution of Candida by Serial Passaging in Host Cells.- Quantifying Receptor-mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells.- Measuring In Vivo Neutrophil Trafficking Responses during Fungal Infection Using Mixed Bone Marrow Chimeras.- Detection of Low Oxygen Microenvironments in a Murine Model of Invasive Pulmonary Aspergillosis Using Pimonidazole.- Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections.- A Murine Model for Chronic A. fumigatus Airway Infections.- Automated Quantitative Analysis of Airway Epithelial Cell Detachment upon Fungal Challenge.