Neuroproteomics

Neuroproteomics: The Methods.- Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue.- Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice.- Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue.- High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics.- Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3).- Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip.- Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes.- Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry.- TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons.- Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures.- Quantitative Analysis of Mass Spectrometry-Based Proteomics Data.- 2D-DIGE as a Tool in Neuroproteomics.- Neuropeptidomics of the Mammalian Brain.- MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides.- A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder.