Protein Lipidation

Determination of Protein S-Acylation State by Enhanced Acyl-Switch Methods.- Detection of Heterogeneous Protein S-Acylation in Cells.- Optimization of Metabolic Labeling with Alkyne-Containing Isoprenoid Probes.- Chemical Proteomic Analysis of S-Fatty Acylated Proteins and their Modification Sites.- Direct Analysis of Protein S-Acylation by Mass Spectrometry.- Enrichment of S-Palmitoylated Proteins for Mass Spectrometry Analysis.- Systematic Screening of Depalmitoylating Enzymes and Evaluation of their Activities by the Acyl-PEGyl Exchange Gel-Shift (APEGS) Assay.- Measuring S-Depalmitoylation Activity In Vitro and In Live Cells with Fluorescent Probes.- Dynamic Radiolabeling of S-Palmitoylated Proteins.- Fluorogenic Assays for the Defatty-Acylase Activity of Sirtuins.- Global Profiling of Sirtuin Deacylase Substrates Using Chemical Proteomic Strategy and Validation by Fluorescent Labeling.- siRNA Knockdown of Mammalian zDHHCs and Validation of mRNA Expression by RT-qPCR.- In Vitro Assays to Monitor the Enzymatic Activities of zDHHC Protein Acyltransferases.- Purification of Recombinant zDHHC Proteins Using an Insect Cell Expression System.- Bioinformatic Identification of Functionally and Structurally Relevant Residues and Motifs in Protein S-Acyltransferases.- SwissPalm 2: Protein S-Palmitoylation Database.- Probing Interaction of Lipid-Modified Wnt Protein and Its Receptors by ELISA.- Biochemical Assays for Ghrelin Acylation and Inhibition of Ghrelin O-Acyltransferase.- In Vitro Analysis of Hedgehog Acyltransferase and Porcupine Fatty Acyltransferase Activities.- Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System.- A Quantitative FRET Assay for the Upstream Cleavage Activity of the Integral Membrane Proteases Human ZMPSTE24 and Yeast Ste24.- Monitoring RhoGDI Extraction of Lipid-Modified Rho GTPases from Membranes using ClickChemistry.- Purification of the Rhodopsin-Transducin Complex for Structural Studies.- Reconstitution of the Rhodopsin-Transducin Complex into Lipid Nanodiscs.