Cell-Derived Matrices Part B

Dr. David Caballero is a senior Research Associate at the 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics at University of Minho (Portugal). Dr. Caballero has broad experience in the fields of biophysics, bioengineering, microfluidics, and nanotechnology. His main research interests include the development of novel bioengineered in vitro models to study the mechanistic determinants of cancer cell invasion. Currently, Dr. Caballero work focuses in the development of innovative multi-organ-on-chip tumor models using engineered native-like 3D biomaterials for drug screening applications. Subhas C. Kundu, PhD, is a Research Coordinator and former European Research Area Chair and Professor at the 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of the University of Minho, Portugal. His research interests include silk biomaterial matrices for biomedical applications, including 3D in vitro cancer models for investigating tumour growth and progression. In addition, he is using natural-based biomaterials for 3D cancer modelling and drug screening. Dr. Rui Reis is Vice-President for Research and Innovation of University of Minho, Portugal, Director of 3B’s Research Group and Director of ICVS/3B´s Associate Laboratory, both of UMinho. He’s the CEO of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, the Coordinator of the Discoveries Centre for Regenerative and Precision Medicine, the Global Past-President of the Tissue Engineering and Regenerative Medicine International Society and the Editor-in-chief of the Journal of Tissue Engineering and Regenerative Medicine. He’s edited 18 books, 10 special issue journals, 280 book chapters and has more than 1225 published works listed on ISI Web of Knowledge, being an inventor of around 70 patents. He’s been awarded many important international prizes, including the TERMIS-EU contributions to the literature Award and the TERMIS-EU Career Achievement Award, and recently the UNESCO- International Life Sciences Award and the IET A. F. Harvey Engineering Research Prize.

PrefaceDavid Caballero, Subhas C. Kundu and Rui L. Reis

Section 1: Tissue decellularization and characterization methods1. Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogelsRabeil Sakina, Aida Llucià-Valldeperas, Ana Henriques Lourenço, Abhishek Harichandan, Sandro Gelsomino, Paul Wieringa, Carlos Mota and Lorenzo Moroni2. Method to decellularize the human chorion membraneLaura P. Frazão, Joana Vieira-de-Castro, Cristina Nogueira-Silva and Nuno M. Neves3. Protocols for decellularization of human amniotic membraneSadjad Khosravimelal, Maryam Momeni, Mahdieh Gholipur, Subhas C. Kundu and Mazaher Gholipourmalekabadi4. Use of supercritical CO2 in soft tissue decellularizationBengisu Topuz, Gülçin Günal, Selcan Guler and Halil Murat Aydin5. Corneal extracellular matrix decellularizationMark Ahearne

Section 2: Decellularized tissues for tissue engineering and disease modelling6. Implementation of a basement membrane invasion assay using mesenteric tissueRitobrata Ghose, Alistair J. Rice, Ernesto Cortes, Upamanyu Ghose, Dariusz Lachowski and Armando del Rio Hernandez7. Decellularized human bone as a 3D model to study skeletal progenitor cells in a natural environmentAna Rita Pereira, Maximilian Rudert and Marietta Herrmann8. Cell sheet tissue engineering for scaffold-free three-dimensional (3D) tissue reconstructionKyungsook Kim, Sophia Bou-Ghannam and Teruo Okano9. Decellularized matrices for tumor cell modelingVirginia Brancato, Maurizio Ventre, Rui L. Reis and Paolo Antonio Netti10. Tissue-derived decellularized extracellular matrices toward cartilage repair and regenerationPiyali Das, Yogendra Pratap Singh, Biman B. Mandal and Samit Kumar Nandi

Section 3: Hybrid systems11.The effect of aligned electrospun fibers and macromolecular crowding in tenocyte cultureDimitrios Tsiapalis, Shubhasmin Rana, Meletios Doulgkeroglou, Stephen Kearns, Jack Kelly, Yves Bayon and Dimitrios I. Zeugolis