Argonaute Proteins

Cloning and Identification of Recombinant-Argonaute Bound Small RNAs using Next-Generation Sequencing.- Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins using SYBR Green-Based qRT-PCR.- Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples.- Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant.- MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans.- cCLIP-Seq: Retrieval of Chimaeric Reads from HITS-CLIP (CLIP-Seq) Libraries.- Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro.- Nucleic Acid-Binding Assay of Argonaute Protein using Fluorescence Polarization.- Reconstitution of RNA Interference Machinery.- Single-Molecule Analysis for RISC Assembly and Target Cleavage.- Profiling Open Chromatin Structure in the Ovarian Somatic Cells using ATAC-Seq.- Assessing miR-451 Activity and Its Role in Erythropoiesis.- Functional Analysis of MicroRNAs in Neurogenesis during Mouse Cortical Development.- Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing.- Genomic Tagging of AG01 using CRISPR/Cas9-Mediated Homologous Recombination.- Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A vade mecum for MINTmap.