Alpha-Synuclein

Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons.- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue.- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons.- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform.- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils.- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection.- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers.- Studying α-Synuclein Conformation by Intact-Cell Crosslinking.- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques.- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein.- CircularDichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes.- Yeast-Based Screens to Target Alpha-Synuclein Toxicity.- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy.- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers.- Monitoring α-Synuclein Proteotoxicity in Drosophila Models.- Detection of α-Synuclein in Biological Samples using Mass Spectrometry.- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation.- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons.- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy.- Expression and Purification of Untagged α-Synuclein.- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson’s Disease.