Odontogenesis

Micro-dissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors.- Establishment of an Immortalized Mouse Bmp2 Knock--Out Dental Papilla Mesenchymal Cell Line.- Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells.- Isolation of Dental Stem Cell Enriched Populations from Continuously Growing Mouse Incisors.- Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentogenesis.- Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-mesenchymal Interactions.- Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, Primary Teeth Stem Cells: Isolation, Characterization and Expansion for Tissue Engineering.- Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparametric Flow Cytometry.- Dental Pulp Stem Cells: Isolation, Characterization, Expansion and Odontoblast Differentiation for Tissue Engineering.- In vitro Analysis of Intramolecular Signaling Events in PDLSCs using Confocal and TIRF Microscopy.- A Mouse Model to Study Reparative Dentinogenesis.- Multiwalled Carbon Nanotubes for Dental Applications.- Peptide-mediated Biomimetic Regrowth of Human Enamel in situ.- Bioengineering Tooth Bud Constructs using GelMA Hydrogel.- Whole-mount In Situ Hybridization of Mouse Embryos Using DIG-labeled RNA Probes.- In Situ Hybridization on Mouse Paraffin Sections Using DIG-labeled RNA Probes.- Methods for in situ Proteins Visualization in Dental Mineralized Tissues.- In Situ Hibridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection.- Immunofluorescence Procedure for Developing Enamel Tissues.- Silver-albumin Tissue Staining Protocol to Visualize Odontogenesis in Whole Embryos.- Isolation of SIBLING Proteins from Bone and Dentin Matrix.- Immunohistochemical Co-localization of Amelogenin and Ameloblastin in Developing Enamel Matrix.- The Expression and Purification of Recombinant Mouse Ameloblastin in e-coli.- Protocols for Studying Formation and Mineralization of Dental Tissues in vivo.- Extraction Protocol for Isolating Dentin Matrix Proteins from Developing Teeth.- Purification of Developing Enamel Matrix Proteins using Preparative SDS PAGE.- Using ImageJ (FIJI) to Analyse and Present X Ray CT Images of Enamel.- Scanning Electron Microscopy (SEM) Methods for Dental Enamel.- Microcomputed Tomography Imaging in Odontogenesis Studies.- Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) for the Examination of Dental Hard Tissues.- Rodent Dental Fluorosis Model--Extraction of Enamel Organ from Rat Incisors.- Three Dimensional Assessment of Crown Size and Eruption Space for Developing Third Molars: Data Collection Techniques Based on Cone-beam Computed Tomography (CBCT).- Protocols to Study Dental Caries In Vitro: Microbial Caries Models.- In vitro Caries Models for the Assessment of Novel Restorative Materials.- Protocols to Study Dental Caries In Vitro: pH Cycling Models.- In Vivo Rodent Models for Studying Dental Caries and Pulp Disease.- Protocol GenoDENT: Implementation of a New NGS Panel for Molecular Diagnosis of Genetic Disorders with Orodental Involvement.- Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues.- Protocols, Methods, and Tools for Genome-wide Association Studies (GWAS) of Dental Traits.- Measurement of Early Childhood Oral Health for Research Purposes: Dental Caries Experience and Developmental Defects of the Enamel in the Primary Dentition.- The Supragingival Biofilm in Early Childhood Caries: Clinical and Laboratory Protocols and Bioinformatics Pipelines Supporting Oral Metagenomics, Metatranscriptomics and Metabolomics Studies of the Oral Microbiome.- Saliva and Gingival Crevicular Fluid (GCF) Collection forBiomarkers Screening.