Programmed Necrosis

Tools in the Art of Studying Necroptosis.- Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules.- Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis.- Distinguishing Necroptosis from Apoptosis.- Methods for Studying TNF-Mediated Necroptosis in Cultured Cells.- Analysis of Necroptosis in Bone Marrow-Derived Macrophages.- Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis.- Detection of MLKL Oligomerization during Programmed Necrosis.- Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation.- Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) during Necroptosis.- Use of RIP1 Kinase Small Molecule Inhibitors in Studying Necroptosis.- Analyzing Necroptosis using a RIPK1 Kinase Inactive Mouse Model of TNF Shock.- Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury.- Assessment of In Vivo Kidney Cell Death: Glomerular Injury.- Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling.- Characterization of the TNFR1-SC using ‘Modified Tandem Affinity Purification’ in Conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS).- Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex.- Analysis of CYLD Proteolysis by Caspase-8 in Bone Marrow-Derived Macrophages.