Clathrin-Mediated Endocytosis

Purification of Clathrin-coated Vesicles from Adult Rat Brain.- Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation.- Probing Endocytosis during the Cell Cycle with Minimal Experimental Perturbation.- Assaying the Contribution of Membrane Tension to Clathrin-mediated Endocytosis.- Identifying Small Molecule Inhibitors of the Clathrin Terminal Domain.- Acute Manipulations of Clathrin-mediated Endocytosis at Presynaptic Nerve Terminals.- Imaging “Hot-wired” Clathrin-mediated Endocytosis.- Real-time Endocytosis Measurements by Membrane Capacitance Recording at Central Nerve Terminals.- Assaying Mutants of Clathrin Mediated Endocytosis in the Fly Eye.- Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-molecule Analysis.- Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in Isolated Plasma Membranes.- SMrT Assay for Real-time Visualization and Analysis of Clathrin Assembly Reactions.- Real-time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System.- Stimulated Emission Depletion (STED) Imaging of Clathrin-mediated Endocytosis in Living Cells.- Measuring Clathrin-coated Vesicle Formation with Single-molecule Resolution.- Cryo-electron Tomography of the Mammalian Synapse.- Quantitative Analysis of Clathrin-mediated Endocytosis in Yeast by Live Cell Fluorescence Microscopy.- Using FM Dyes to Monitor Clathrin-mediated Endocytosis in Primary Neuronal Culture.