Procedure Manual for the Diagnosis of Intestinal Parasites

Donald L. Price, Ph.D., received his doctorate in zoology from the University of Maryland, with a specialty in Medical Zoology. He has broad experience as a scientist, consultant, and educator. For 22 years, Dr. Price served in the United States Army, first as a pilot in WWII, and for 18 years as a Medical Zoologist (parasitology) in the Medical Service Corps. He retired in 1968 as a Lieutenant Colonel.

Introduction
Survival of Trophozoites and Cysts in Unpreserved Fecal Specimens
Collecting/Preserving Solutions
The Use of Collecting/Preserving Solutions
The Quality of Preserved Eggs and Parasites
Background of the MIF-Type Collecting/Preserving Solutions
Environmental Considerations
Application of Collecting/Preserving Methods
The Specimen
Fecal Specimens in the Clinical Laboratory
Factors Having an Impacting on Specimen Collection
Procedure for Specimen Collection for Parasitology
Factors Having an Impact on Specimen Preparation
Written Instructions for Specimen Collection.
Instructions for Collecting Fecal Specimens
The Microscope and Examination of the Specimen
The Microscope and Microscopy
Using Their Refractive Index to Find Parasites and Eggs
Eyepiece Micrometer
Selection of Lenses
Preparing Wet-Mount Slides from the Preserved Specimen for Microscopic Examination
Concentration Methods
Introduction
Use of the Centrifuge for Concentration Methods
Flotation Methods
Sedimentation Methods
Stains and Staining Methods
Stains and Staining Methods
Preparing Permanently Stained Fecal Films
Preparing Fecal Films for Permanent Staining
Staining Methods for Preparing Permanently Stained Fecal Films from Specimens Fixed in PVA
Staining Methods for Preparing Permanently Stained Fecal Films from Specimens Fixed in SAF, PIF, or MIF
Reusing and Changing Solutions in the Staining Setup
Application of Methods
Comparison of Collecting/Preserving Solutions.
Selecting Methods for the Laboratory
Special Methods
Acid-Fast Stains
Preparing Temporary Wet Mounts
Egg Count Procedures
Post-Treatment Examinations
Introduction to the Parasites
Parasites Identified by Examining Fecal Preparations
Helminth Parasites in General
Protozoan Parasites in General
Source of Infection and Modes of Transmission
The Helminths
Diagnosis of Helminth Infections
Trematode Eggs
Cestode Eggs
Nematode Eggs
Nematode Juveniles
Introduction to the "Key to Helminth Eggs Found in Feces"
Photomicrographs of Helminth Eggs
Key to Helminth Eggs Found in Feces
The Protozoa
Structure of the Intestinal Protozoa
Structure of the Intestinal Amoebae
Structure of the Intestinal Flagellates
Structure of the Intestinal Ciliates
Structure of the Intestinal Coccidia
The Intestinal Amoebae
Introduction to the "Key for Differentiating Species of Intestinal Amoebae in Fecal Specimens Preserved in PIF or MIF"
Key for Differentiating Species of Intestinal Amoebae in Specimens Preserved in PIF or MIF
The Intestinal Flagellates
The Intestinal Ciliates
The Intestinal Coccidia
Protozoa of Uncertain Taxonomic Position
Photomicropgraphs of Intestinal Protozoa
Nonhuman Parasites and Structures that Mimic Parasites
Structures Resembling Protozoa
Structures Resembling Helminth Eggs
Spurious Infections with Eggs and Cysts of Animals
Appendices
Technical Information
Glossary
References
Index