Protein Expression in Mammalian Cells

Why Proteins in Mammalian Cells?.- Large Scale Transfection of Mammalian Cells.- Selection of High Expressing Mammalian Cells by Surface Display of Reporters.- Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles.- Transfection of Difficult-to-Transfect Primary Mammalian Cells.- Stable Protein Expression in Mammalian Cells Using Baculoviruses.- Using Matrix Attachment Regions to Improve Recombinant Protein Production.- Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells.- Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells.- Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells.- Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker.- Tools for Co-Producing Multiple Proteins in Mammalian Cells.- Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases.- Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA.- Methods for Constructing Clones for Protein Expression in Mammalian Cells.- Optimizing Transient Recombinant Protein Expression in Mammalian Cells.