Ubiquitin Family Modifiers and the Proteasome

Three Decades of Studies to Understand the Functions of the Ubiquitin Family.- Structure and Evolution of Ubiquitin and Ubiquitin-Related Domains.- Deciphering Tissue-Specific Ubiquitylation by Mass Spectrometry.- Analysis of Cellular SUMO and SUMO-Ubiquitin Hybrid Conjugates.- Recombinant Reconstitution of Sumoylation Reactions In vitro.- Reconstitution of SUMO-Dependent Ubiquitylation In vitro.- Detection and Analysis of FAT10 Modification.- Isolation of NEDDylated Proteins in Human Cells.- The ISG15 Conjugation System.- Pupylation: Proteasomal Targeting by a Protein Modifier in Bacteria.- Role of UbF Family Modifiers and their Binding Proteins in Cell Signaling.- Isolation of Ubiquitylated Proteins using Tandem Ubiquitin-Binding Entities (TUBEs).- Using Linkage-Specific Monoclonal Antibodies to Analyze Cellular Ubiquitination.- Measuring Rates of Ubiquitin Chain Formation as a Functional Readout of Ligase Activity.- Synthesis and Analysis of K11-Linked Ubiquitin Chains.- Synthesis and Analysis of Linear Ubiquitin Chains.- Detection and Quantitation of SUMO Chains by Mass Spectrometry.- Bioinformatical Detection of Recognition Factors for Ubiquitin and SUMO.- Surface Plasmon Resonance to Measure Interactions of UbFs with Their Binding Partners.- Identifying and Studying Ubiquitin Receptors by NMR.- Exploring the Role of p97 and its UBX-Domain Cofactors Through Identification of Their Interacting Proteins.- Assembly and Function of the Proteasome.- Using Native Gel Electrophoresis and Phosphofluoroimaging to Analyse GFP-tagged Proteasomes.- Disulfide Engineering to Map Subunit Interactions in the Proteasome and Other Macromolecular Complexes.- Using DNA Damage Sensitivity Phenotypes to Characterize Mutations Affecting Proteasome Function.- Analysing Properties of Proteasome Inhibitors using Kkinetic and X-Ray Crystallographic Studies.- Immunoproteasome-Specific Inhibitors and Their Application.- Binding of Ubiquitin Conjugates to Proteasomes as Visualized with Native Gels.- Affinity Purification of Mmammalian 26S Proteasomes Using an Ubiquitin-Like Domain.- Using siRNA Techniques to Dissect Proteasome Assembly Pathways in Mammalian Cells.- Reconstitution of PA700, the 19S Regulatory Particle, from Purified Precursor Complexes.- Cellular Responses to Misfolded Proteins and Protein Aggregates.- Live-Cell Imaging of Ubiquitin-Proteasome System Function.- Analysis of Chaperone-assisted Ubiquitylation.- Use of CPY* and its Derivatives to Study Protein Quality Control in Various Cell Compartments.- Assays to Measure ER-Associated Degradation in Yeast.- SDS-PAGE Techniques to Study Ubiquitin-Like Conjugation Systems in Yeast Autophagy.- Analysis of Ubiquitin-Dependent Proteolysis in C. elegans.- Structural Insights into Functional Modes of Proteins Involved in Ubiquitin Family Pathways.- Identification and Application of NEDD8 E1 Inhibitors.- Formation of Ubiquitin Dimers via Azide Alkyne Click Reaction.- Synthesis of Atypical Diubiquitin Chains.- TIPI - TEV Protease-Mediated Induction of Protein Instability.- PROTAC-Induced Proteolytic Targeting.