Proteomic Methods in Neuropsychiatric Research (eBook)

Preface – Proteomic studies in psychiatric disease and neurological disorders.- Part 1: REVIEWS.- Application of proteomic techniques for improved stratification and treatment of schizophrenia patients.- Multiplexing biomarker methods, proteomics and considerations for Alzheimer’s disease.- What have proteomic studies taught us about novel drug targets in autism?.- Application of proteomic approaches to accelerate drug development for psychiatric disorders.- Proteomic biomarker identification in cerebrospinal fluid for leptomeningeal metastases with neurological complications.- Connecting brain proteomics with behavioural neuroscience in translational animal models of neuropsychiatric disorders.- LC-MSE for qualitative and quantitative proteomicstudies of psychiatric disorders.- The utility of multiplex assays for identification of proteomic signatures in psychiatry.- Part 2: PROTOCOLS.- Blood sampling and preparation procedures for proteomic biomarker studies of psychiatric disorders.- Multiplex immunoassay profiling of serum in psychiatric disorders.- Sequential immunopreciptation of secretory vesicle proteins from biosynthetically-labelled cells.- 2d gel electrophoresis of insulin secretory granule proteins from biosynthetically-labelled pancreatic islets.- Two-dimensional gel electrophoresis: a reference protocol.- A two-dimensional difference gel electrophoresis (2D-DIGE) protocol for studies of neural precursor cells.- Identifying biomarker candidates in the blood plasma or serum proteome.- Selective reaction monitoring mass spectrometry for quantitation of glycolytic enzymes in post-mortem brain samples.- A selected reaction monitoring mass spectrometry protocol for validation of proteomic biomarker candidates in studies of psychiatric disorders.- Application of iTRAQ shotgun proteomics for measurement of brain proteins in studies of psychiatric disorders.- Co-immunoprecipitation for deciphering protein interactomes.- Sequential co-immunopreciptation and immunoblot approach to determine oligomerisation of G-protein coupled receptors.- A protocol for producing the maternal low protein rat model: a tool for pre-clinical proteomic studies.- A clinical study protocol to identify serum biomarkers predictive of response to antipsychotics in schizophrenia patients.- Generation of the acute phencyclidine rat model for proteomic studies of schizophreniaA protocol for generation of a corticosterone model of psychiatric disorders.- MK-801-treated oligodendrocytes as a cellular model to study schizophrenia.- Combining patient-reprogrammed neural cells and proteomics as a model to study psychiatric disorders.- SILAC mass spectrometry profiling: a psychiatric disorder perspective.- Preparation of peripheral blood mononuclear cells (PBMCs) as a model for proteomic studies psychiatric disorders.- Proteomic profiling of skin fibroblasts as a model of schizophreniaProteomic profiling of the pituitary gland in studies of psychiatric disorders.- Development of an assay for measuring proprotein-conversion activity on a multiplex magnetic bead-based array platform.- Phenotyping multiple subsets of immune cells in situ in formalin-fixed, paraffin-embedded tissue sections.- Lab-on-a-chip proteomic assays for psychiatric disorders.- Development of a user-friendly app for testing blood coagulation status in schizophrenia patients.- Part 3:FUTURE PERSPECTIVES.- Proteomic approaches to enable point-of-care testing and personalized medicine for psychiatric disorders.