Plasmodesmata
Humana Press Inc. (Verlag)
978-1-4939-4643-3 (ISBN)
Practical and easy to use, Plasmodesmata: Methods and Protocols delivers detailed information about specific practical procedures in order to enable their reproduction and application in PD-related research in laboratories around the world.
Plasmodesmata: Channels for Intercellular Signaling During Plant Growth and Development.- Plasmodesmata: Channels for Viruses on the Move.- Imaging Plasmodesmata with High Resolution Scanning Electron Microscopy.- Preparative Methods for Imaging Plasmodesmata at Super-Resolution.- Isolation of Plasmodesmata from Arabidopsis Suspension Culture Cells.- Immunofluorescence Detection of Callose Deposition around Plasmodesmata Sites.- Imaging Callose at Plasmodesmata Using Aniline Blue: Quantitative Confocal Microscopy.- Localization of Fluorescently-Tagged Protein to Plasmodesmata by Correlative Light and Electron Microscopy.- Quantification of Plant Cell Coupling with Live-Cell Microscopy.- Drop-ANd-See: A Simple, Real-Time, and Non-Invasive Technique for Assaying Plasmodesmal Permeability.- Mapping Symplasmic Fields at the Shoot Apical Meristem Using Iontophoresis and Membrane Potential Measurements.- Analysis of the Conductivity of Plasmodesmata by Microinjection.- Investigating Plasmodesmata Genetics with Virus-Induced Gene Silencing and an Agrobacterium-Mediated GFP Movement Assay.- Probing Plasmodesmata Function with Biochemical Inhibitors.- GAL4 Transactivation-Based Assay for the Detection of Selective Intercellular Protein Movement.- Techniques for Assessing the Effects of Pharmacological Inhibitors on Intercellular Protein Movement.- Probing Protein Targeting to Plasmodesmata Using Fluorescence Recovery after Photo-Bleaching.- The Tracking of Intercellular Small RNA Movement.- Analysis of the Role of Myosins in Targeting Proteins to Plasmodesmata.- Pumilio-Based RNA In Vivo Imaging.- In Vivo RNA Labeling Using MS2.
Erscheinungsdatum | 23.03.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 1217 |
Zusatzinfo | 39 Illustrations, color; 14 Illustrations, black and white; X, 346 p. 53 illus., 39 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Schlagworte | cell signaling • Cell-to-cell communication • Macromolecular trafficking • PD conductivity and regulation • PD imaging • Plasmodesmata (PD) • Virus models |
ISBN-10 | 1-4939-4643-9 / 1493946439 |
ISBN-13 | 978-1-4939-4643-3 / 9781493946433 |
Zustand | Neuware |
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