Light Microscopy

Introduction to Modern Methods in Light Microscopy.- Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM).- Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy.- Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis.- Imaging of Brain Slices with a Genetically Encoded Voltage Indicator.- FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells.- Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum.- Targeted Ablation Using Laser Nanosurgery.- Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells.- STED Imaging in Drosophila Brain Slices.- Two-Color Total Internal Refection Fluorescence Microscopy of Exocytosis in Endocrine Cells.- Optical Coherence Microscopy.- Designing Image Analysis Pipelines in Light Microscopy: A Rationale Approach.- Automated Analysis of Intracellular Dynamic Processes.- Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells.- Analysis of Protein Kinetics using Fluorescence Recovery After Photobleaching (FRAP).- Fluorescence Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening.