Detection of Blotted Proteins

Western Blotting: Origin and Ascent of the Species.- Methods to Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic Evaluation.- Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose.- Detection of Blotted Proteins: Not all Blockers are Created Equal.- Protein Stains to Detect Antigen on Membranes.- Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting.- Rapid, Antibody-free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation.- Use of Non-Radioactive Detection Method for North- and South-Western Blot.-  Immunoblotting using Radiolabeled Reagents for Detection.- Immunoblotting of Antigens: Whole, Strip and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique.- Detection of Protein Carbonyls by Means of Biotin Hydrazide –Streptavidin Affinity Methods.- Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel.- Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays.- Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots.- Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL-plex Immunoblotting in a Standard Proteomics Workflow.- Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot blots and Immunohistochemistry.- Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane.- Sequential use of Immunoblots for Characterization of Autoantibody Specificities.- Nanogold Immunodetection DetectionSystems for the Identification of Autoantigens by Western Blotting.- Application of Intermittent Microwave Irradiation to Western Blot Analysis.- Visualization of Unstained Protein Bands on PVDF.- Multiplexed Fluorescent Immunodetection Using Low Auto-Fluorescence Immobilon®-FL Membrane.- Cold Microwave-Enabled Protein Detection and Quantification.- TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics.- Analysis of Electroblotted Proteins by Mass Spectrometry.- On Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions.- Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore.- Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane.- Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta.- Non-stripping “Rainbow” and Multiple Antigen Detection (MAD) Western Blotting. - Supported Molecular Matrix Electrophoresis.- Parafilm-M®, an Available Cost Effective Alternative for Immuno-blot Pouches.- Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes.- Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva.- A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB.- Other Notable Methods of Membrane Protein Detection: A Brief Review.- Nitrocellulose Membrane: The New Canvas.- Invisible Ink Marking in ECL Membrane Assays.